Synbiotic effects of Lactobacillus rhamnosus fermented with different prebiotics on the digestive enzyme activities, SCFAs and intestinal flora of Macrobrachium rosenbergii in vitro

• Published in: Aquaculture reports 2024-10, Vol.38, p.102303, Article 102303
• Main Authors: Li, Zhengzhong, Liu, Mingyang, Zhou, Qunlan, Sun, Cunxin, Zheng, Xiaochuan, Chen, Jianming, Liu, Bo, Liu, Shijie, Liu, Shengli, Wang, Aiming
• Format: Article
• Language: English
• Subjects: In vitro fermentation; Intestinal flora; Lactobacillus rhamnosus; Macrobrachium rosenbergii; Synbiotics
• ISSN: 2352-5134; 2352-5134
• DOI: 10.1016/j.aqrep.2024.102303

Synbiotics are combinations of probiotics and prebiotics. It has been shown to be beneficial to aquatic animals. In this study, Lactobacillus rhamnosus was cultured with different prebiotics as the sole carbon source, including inulin, acanthopanax senticosus polysaccharide (ASPS), chitosan oligosaccharide (COS), saposhnikovia divaricata polysaccharide (SDP), polysaccharide of atractylodes macrocephala koidz (PAMK), astragalus polysaccharides (APS), Yu-Ping-Feng polysaccharide (YPFP), xylooligosaccharides (XOS) and mannose (MAN). Screening and gradient experiments revealed that the suitable prebiotics and concentrations were: 2 % MAN, 3 % APS, 3 % YPFP, 4 % PAMK, 3 % SDP. L. rhamnosus (108 CFU/mL) and suitable prebiotics were inoculated into the media supplemented with the intestinal chyme of Macrobrachium rosenbergii. Based on the preliminary screening experiment with the gradients. The in vitro fermentation experiment comprised seven groups: a control group (CT); L. rhamnosus group (LGG); L. rhamnosus + 2 % MAN group (LGG+MAN); L. rhamnosus + 3 % APS group (LGG+APS); L. rhamnosus + 3 % YPFP group (LGG+YPFP); L. rhamnosus + 4 % PAMK group (LGG+PAMK); L. rhamnosus + 3 % SDP group (LGG+SDP). The in vitro fermentation results revealed that the amylase activity was significantly increased in LGG+PAMK, LGG+SDP and LGG+APS groups than in the CT group (P