Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication

• Published in: iScience 2024-01, Vol.27 (1), p.108684-108684, Article 108684
• Main Authors: Zhang, Chu, Wu, Hui, Feng, Hao, Zhang, Yong-An, Tu, Jiagang
• Format: Article
• Language: English
• Subjects: Fish culture; Virology
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2023.108684

Viral inclusion bodies (VIBs) are subcellular structures required for efficient viral replication. How type II grass carp reovirus (GCRV-II), the mainly prevalent strain, forms VIBs is unknown. In this study, we found that GCRV-II infection induced punctate VIBs in grass carp ovary (GCO) cells and that non-structural protein 38 (NS38) functioned as a participant in VIB formation. Furthermore, VP56 and VP35 induced VIBs and recruited other viral proteins via the N-terminal of VP56 and the middle domain of VP35. Additionally, we found that the newly synthesized viral RNAs co-localized with VP56 and VP35 in VIBs during infection. Taken together, VP56 and VP35 induce VIB formation and recruit other viral proteins and viral RNAs to the VIBs for viral replication, which helps identify new targets for developing anti-GCRV-II drugs to disrupt viral replication. [Display omitted] •GCRV-II infection induces viral inclusion bodies (VIBs) formation•VP56 and VP35 induce VIBs formation•The N-terminal of VP56 and the middle domain of VP35 recruit viral proteins to VIBs•Newly synthesized viral RNAs are localized to the VIBs Virology; Fish culture