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Isolation and genomic characterization of a Dehalococcoides strain suggests genomic rearrangement during culture
We have developed and characterized a bacterial consortium that reductively dechlorinates trichloroethene to ethene. Quantitative PCR analysis for the 16S rRNA and reductive dehalogenase genes showed that the consortium is highly enriched with Dehalococcoides spp. that have two vinyl chloride reduct...
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Published in: | Scientific reports 2017-05, Vol.7 (1), p.2230-11, Article 2230 |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | We have developed and characterized a bacterial consortium that reductively dechlorinates trichloroethene to ethene. Quantitative PCR analysis for the 16S rRNA and reductive dehalogenase genes showed that the consortium is highly enriched with
Dehalococcoides
spp. that have two vinyl chloride reductive dehalogenase genes,
bvcA
and
vcrA
, and a trichloroethene reductive dehalogenase gene,
tceA
. The metagenome analysis of the consortium by the next generation sequencer SOLiD 3 Plus suggests that a
Dehalococcoides
sp. that is highly homologous to
D. mccartyi
195 and equipped with
vcrA
and
tceA
exists in the consortium. We isolated this
Dehalococcoides
sp. and designated it as
D. mccartyi
UCH-ATV1. As the growth of
D. mccartyi
UCH-ATV1 is too slow under isolated conditions, we constructed a consortium by mixing
D. mccartyi
UCH-ATV1 with several other bacteria and performed metagenomic sequencing using the single molecule DNA sequencer PacBio RS II. We successfully determined the complete genome sequence of
D. mccartyi
UCH-ATV1. The strain is equipped with
vcrA
and
tceA
, but lacks
bvcA
. Comparison with tag sequences of SOLiD 3 Plus from the original consortium shows a few differences between the sequences. This suggests that a genome rearrangement of
Dehalococcoides
sp. occurred during culture. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-017-02381-0 |