Different inflammatory responses are associated with Ureaplasma parvum-induced UTI and urolith formation

Epidemiologic studies show a strong association between Ureaplasmas and urogenital tract disease in humans. Since healthy humans can be colonized with Ureaplasmas, its role as a pathogen remains controversial. In order to begin to define the role of the host in disease, we developed a rodent model o...

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Bibliographic Details
Published in:BMC infectious diseases 2009-01, Vol.9 (1), p.9-9, Article 9
Main Authors: Reyes, Leticia, Reinhard, Mary, Brown, Mary B
Format: Article
Language:English
Subjects:
Animals
Bacterial infections
Bacteriuria - etiology
Bacteriuria - immunology
Calculi, Urinary
Complications and side effects
Cytokines - urine
Disease Models, Animal
Female
Health aspects
Immune response
Inflammation - immunology
Kidney - microbiology
Kidney - pathology
Mycoplasmatales
Rats
Rats, Inbred F344
Risk factors
Ureaplasma - pathogenicity
Ureaplasma Infections - complications
Ureaplasma Infections - immunology
Urinary Bladder - microbiology
Urinary Bladder - pathology
Urinary tract infections
Urolithiasis - etiology
Urolithiasis - immunology
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Summary:Epidemiologic studies show a strong association between Ureaplasmas and urogenital tract disease in humans. Since healthy humans can be colonized with Ureaplasmas, its role as a pathogen remains controversial. In order to begin to define the role of the host in disease, we developed a rodent model of urinary tract infection (UTI) using Fischer 344 (F344) rats. Animals were inoculated with sterile broth, 10(1), 10(3), 10(5), 10(7), or 10(9) log CFU of a rat-adapted strain of Ureaplasma parvum. Infected animals exhibited two distinct profiles, asymptomatic UTI and UTI complicated with struvite urolithiasis. Inoculum dose of U. parvum affected the incidence of UTI, and 50% to 57% of animals inoculated with >or= 10(7) CFU of U. parvum remained infected (p < 0.04). However, inoculum dose did not influence immune response to U. parvum. Asymptomatic UTI was characterized by a minimal immune response that was predominantly monocytic and lymphocytic, with limited lesions, and elevated urinary levels of IFN-gamma, IL-18 and MCP-1 (P
ISSN:1471-2334
1471-2334
DOI:10.1186/1471-2334-9-9