Protective Effect of Celastrus orbiculatus Thunb Extract on Cultured Neuroglial Cells Damaged by Manganese Dioxide, a Parkinsonism Inducer

• Published in: Korean journal of clinical laboratory science 2020-06, Vol.52 (2), p.150-157
• Main Author: Seo, Young-Mi
• Format: Article
• Language: English
• Subjects: antioxidative effect; cytotoxicity; manganese dioxide; parkinsonism
• ISSN: 1738-3544; 2288-1662
• DOI: 10.15324/kjcls.2020.52.2.150

The protective effects of a Celastrus orbiculatus Thunb (CO) extract against manganese dioxide (MnO2)-induced cytotoxicity in cultured C6 glioma cells were examined. This study assessed the antioxidative effects, including the suppressive ability of lipid peroxidation (LP), the inhibitory ability of xanthine oxidase (XO), and the cell viability. MnO2 decreased the cell viability remarkably in a dose-dependent manner. The XTT50 value was determined to be 146.7 μM in these cultures. The cytotoxicity of MnO2 was calculated to be mid-toxic using Borenfreund and Puerner’s toxic criteria. Kaempferol (KAE) increased the cell viability damaged by MnO2-induced cytotoxicity significantly. Regarding the protective effects of the CO extract on MnO2-induced cytotoxicity, the CO extract increased cell viability significantly compared to the MnO2-treated group. The CO extract also had inhibitory abilities against lipid peroxidation (LP) and xanthine oxidase (XO). From these findings, oxidative stress is involved in the cytotoxicity of MnO2. The CO extract effectively blocked the cytotoxicity induced by MnO2 via its antioxidative effects. Conclusively, natural resources, such as the CO extract, might be a useful agent for the diminution or improvement of the heavy metal cytotoxicity correlated with disease through oxidative stress, such as MnO2, a Parkinsonism inducer.