Antimicrobial resistance and its relationship with antimicrobial use on Austrian dairy farms

• Published in: Frontiers in veterinary science 2023-07, Vol.10, p.1225826-1225826
• Main Authors: Werner, Thomas, Käsbohrer, Annemarie, Wasner, Barbara, Köberl-Jelovcan, Sandra, Vetter, Sebastian G, Egger-Danner, Christa, Fuchs, Klemens, Obritzhauser, Walter, Firth, Clair L
• Format: Article
• Language: English
• Subjects: antibiotics; antimicrobial resistance; calves; cattle; ESBL; Escherichia coli; Veterinary Science
• ISSN: 2297-1769; 2297-1769
• DOI: 10.3389/fvets.2023.1225826

The aim of this study was to investigate the prevalence of ESBL/AmpC-producing and the resistance pattern of commensal , as well as the link between the use of antibiotics (AMU) and the occurrence of resistance in on Austrian dairy farms. AMU data from 51 farms were collected over a one-year period in 2020. Fecal samples were collected from cows, pre-weaned and weaned calves in 2020 and 2022. Samples were then analyzed using non-selective and selective agar plates, isolates were confirmed by MALDI-TOF analysis. Broth microdilution was used for antimicrobial susceptibility testing. The AMU of each farm was quantified as the number of Defined Daily Doses (nDDD ) and Defined Course Doses (nDCD ) per cow and year. Cephalosporins (mean 1.049; median 0.732 DDD /cow/year) and penicillins (mean 0.667; median 0.383 DDD /cow/year) were the most frequently used antibiotics on these farms, followed by tetracyclines (mean 0.275; median 0.084 DDD /cow/year). In 2020, 26.8% of the isolated were resistant to at least one antibiotic class and 17.7% of the isolates were classified as multidrug resistant (≥3 antibiotic classes). Out of 198 isolates, 7.6% were identified as extended-spectrum/AmpC beta-lactamase (ESBL/AmpC) producing . In 2022, 33.7% of isolates showed resistance to at least one antibiotic and 20.0% of isolates displayed multidrug resistance. Furthermore, 29.5% of the samples carried ESBL/AmpC-producing . In 2020 and 2022, the most frequently determined antibiotic resistances among commensal isolates were to tetracyclines, sulfonamides and penicillins. In addition, pre-weaned calves had the highest resistance rates in both years. Statistical analyses showed a significant association between low and high use AMU classifications for penicillins (in nDDD /cow/year) and their respective resistance among commensal isolates in 2020 (  = 0.044), as well as for sulfonamide/trimethoprim (  = 0.010) and tetracyclines (  = 0.042). A trend was also noted between the total amount of antibiotics used on farm in 2020 (by nDDD /cow/year) and multidrug resistances in commensal isolated on farm that year (  = 0.067). In conclusion, the relationship between AMU and antimicrobial resistance (AMR) on dairy farms continues to be complex and difficult to quantify.