Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1

• Published in: Journal of Integrative Agriculture 2013-04, Vol.12 (4), p.638-645
• Main Authors: MA, Dong-fang, HOU, Lu, TANG, Ming-shuang, WANG, Hai-ge, LI, Qiang, JING, Jin-xue
• Format: Article
• Language: English
• Subjects: molecular mapping; Psathyrostachys huashanica Keng; Puccinia striiformis f. sp. tritici; resistance genes; SSR标记; wheat cultivar; wheat cultivar Puccinia striiformis f. sp. tritici resistance genes molecular mapping Psathyrostachys huashanica Keng; 分子标记辅助选择; 小麦白粉病; 抗条锈病基因; 染色体位置; 标记基因型; 简单重复序列; 遗传分析
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(13)60271-3

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14-4-6-1 has all stage resistance. To identify stripe rust resistance genes, the segregating populations were developed from the cross between H9014-14-4-6-1 and Mingxian 169 (a wheat cultivar susceptible to all Pst races identified in China). The seedlings of the parents and F1 plants, F2, F3 and BC1 generations were tested with Pst races under controlled greenhouse conditions. Two genes for resistance to stripe rust were identified, one dominant gene conferred resistance to SUN11-4, temporarily designated YrH9014 and the other recessive gene conferred resistance to CYR33. The bulked segregant analysis and simple sequence repeat (SSR) markers were used to identify polymorphic markers associated with YrH9014. Seven polymorphic SSR markers were used to genotype the F2 population inoculated with SUN11-4. A linkage map was constructed according to the genotypes of seven SSR markers and resistance gene. The molecular map spanned 24.3 cM, and the genetic distance of the two closest markers Xbarc13 and Xbarc55 to gene locus was 1.4 and 3.6 cM, respectively. Based on the position of SSR marker, the resistance gene YrH9014 was located on chromosome arm 2BS. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xbarc13 indicated that YrH9014 was located on chromosome 2B. Based on chromosomal location, the reaction patterns and pedigree analysis, YrH9014 should be a novel resistance gene to stripe rust. This new gene and flanking markers got from this study should be useful for marker-assisted selection (MAS) in breeding programs for stripe rust.