Chlamydia trachomatis L2c Infection in a Porcine Model Produced Urogenital Pathology and Failed to Induce Protective Immune Responses Against Re-Infection

• Published in: Frontiers in immunology 2020-10, Vol.11, p.555305-555305
• Main Authors: De Clercq, Evelien, Van Gils, Matthias, Schautteet, Katelijn, Devriendt, Bert, Kiekens, Celien, Chiers, Koen, Van Den Broeck, Wim, Cox, Eric, Dean, Deborah, Vanrompay, Daisy
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; Antibody Formation - immunology; Biopsy; Chlamydia Infections - veterinary; Chlamydia trachomatis; Female; genital infection; Immunity, Mucosal; Immunohistochemistry; Immunology; large animal model; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - metabolism; Lymphocytes - immunology; Lymphocytes - metabolism; lymphogranuloma venereum; Male; re-infection; Reinfection; Swine; Swine Diseases - immunology; Swine Diseases - microbiology; Swine Diseases - pathology
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2020.555305

The current study was designed to evaluate the pathogenesis, pathology and immune response of female genital tract infection with L2c, the most recently discovered lymphogranuloma venereum strain, using a porcine model of sexually transmitted infections. Pigs were mock infected, infected once or infected and re-infected intravaginally, and samples were obtained for chlamydial culture, gross and microscopic pathology, and humoral and cell-mediated immunity. Intravaginal inoculation of pigs with this bacterium resulted in an infection that was confined to the urogenital tract, where inflammation and pathology were caused that resembled what is seen in human infection. Re-infection resulted in more severe gross pathology than primary infection, and chlamydial colonization of the urogenital tract was similar for primary infected and re-infected pigs. This indicates that primary infection failed to induce protective immune responses against re-infection. Indeed, the proliferative responses of mononuclear cells from blood and lymphoid tissues to strain L2c were never statistically different among groups, suggesting that -specific lymphocytes were not generated following infection or re-infection. Nevertheless, anti-chlamydial antibodies were elicited in sera and vaginal secretions after primary infection and re-infection, clearly resulting in a secondary systemic and mucosal antibody response. While primary infection did not protect against reinfection, the porcine model is relevant for evaluating immune and pathogenic responses for emerging and known strains to advance drug and/or vaccine development in humans.