Curcumin improved liposomal mitomycin-induced cell toxicity in bladder cancer cell

Objective (s): This study aimed at investigation of preparation and stability study of Mitomycin and Mitomycin loaded nanoliposems and efficacy of the combination of Mitomycin -loaded nanoliposomes and Curcumin versus Mitomycin in suppressing HTB-9 and L929 cell lines, in vitro.Materials and Methods...

Full description

Saved in:
Bibliographic Details
Published in:Nanomedicine journal 2018-10, Vol.5 (4), p.235-244
Main Authors: Farjad Amanolahi, Ali Mohammadi, Reza Kazemi oskuee, Leila Golami, Bizhan Malaekeh-Nikouei
Format: Article
Language:English
Subjects:
Combination therapy
Curcumin
Cytotoxicity
Liposome
Mitomycin
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective (s): This study aimed at investigation of preparation and stability study of Mitomycin and Mitomycin loaded nanoliposems and efficacy of the combination of Mitomycin -loaded nanoliposomes and Curcumin versus Mitomycin in suppressing HTB-9 and L929 cell lines, in vitro.Materials and Methods: An HPLC method was validated based on Q2 (R1) International Conference on Harmonization (ICH) guideline for determination of Mitomycin in pharmaceutical samples. Soybean phosphatidylcholine (SPC) or hydrogenated soybean phosphatidylcholine (HSPC) mixed with cholesterol at 2:1 molar ratio respectively in two different groups to prepare Mitomycin nanoliposomes. Cell toxicity of free Mitomycin, Mitomycin loaded liposomes and curcumin was measured using MTT assay in bladder cancer cell line for treatment group and Fibroblast-like cell as control group. Also, propidium iodide staining was done to determine the level of DNA fragmentation.Results: The validated HPLC method showed stability based on the ICH guideline (n=3 and RSD
ISSN:2322-3049
2322-5904
DOI:10.22038/nmj.2018.05.00008