Hyperbaric oxygenation and the genic expression related to oxidative stress in the heart of mice during intestinal ischemia and reperfusion

Abstract Purpose: To investigate the effects of hyperbaric oxygenation (HBO) on intestinal ischemia and reperfusion (IR) injury, we evaluated the expression of 84 genes related to oxidative stress and the antioxidant response in mouse hearts. Methods: Four groups were subjected to 60 minutes of inte...

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Bibliographic Details
Published in:Acta cirúrgica brasileira 2017-11, Vol.32 (11), p.913-923
Main Authors: Somaio Neto, Frederico, Ikejiri, Adauto Tsutomo, Bertoletto, Paulo Roberto, Chaves, José Carlos, Teruya, Roberto, Fagundes, Djalma José
Format: Article
Language:English
Subjects:
Gene Expression
Mice
Oxidative Stress
Reperfusion Injury, Hyperbaric Oxygenation
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Summary:Abstract Purpose: To investigate the effects of hyperbaric oxygenation (HBO) on intestinal ischemia and reperfusion (IR) injury, we evaluated the expression of 84 genes related to oxidative stress and the antioxidant response in mouse hearts. Methods: Four groups were subjected to 60 minutes of intestinal ischemia followed by 60 minutes of reperfusion: IRG, ischemia and reperfusion group without HBO; HBO-IG, which received HBO during ischemia; HBO-RG, which received HBO during reperfusion; and HBO-IRG, which received HBO during ischemia and reperfusion. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes. The (RT-qPCR) method was applied. Genes with expression levels three times below or above the threshold cycle were considered significantly hypoexpressed or hyperexpressed, respectively (Student’s t-test p
ISSN:0102-8650
1678-2674
DOI:10.1590/s0102-865020170110000003