Study of humoral immune response in mammals immunized with Plasmodium falciparum antigenic preparations

Six Plasmodium falciparum protein fractions, isolated under reducing conditions, were used to immunize mice, rabbits and the squirrel monkey Saimiri sciureus. Five or seven subcutaneous injections of each antigenic preparation, in conjunction with Freund's complete or incomplete adjuvants, were...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 1992, Vol.87 Suppl 3 (suppl 3), p.429-434
Main Authors: Cubas, A B, Cunha, F, Nitcheu, I K, Rolland, L, Ciceron, L, Carvalho, A B, Furtado, A F, Monjour, L
Format: Article
Language:English
Subjects:
Animals
Antibodies, Protozoan - biosynthesis
Antibodies, Protozoan - immunology
Antigens, Protozoan - immunology
Female
Immune Sera
Immunization, Secondary
Immunoglobulin G - immunology
Immunoglobulin M - immunology
in vitro parasite inhibition
Malaria Vaccines - immunology
Male
Mice
Mice, Inbred BALB C
PARASITOLOGY
Plasmodium falciparum
Plasmodium falciparum - growth & development
Plasmodium falciparum - immunology
protein fractions
Protozoan Proteins - immunology
Rabbits
rhoptry proteins
Saimiri
TROPICAL MEDICINE
Vaccination
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Summary:Six Plasmodium falciparum protein fractions, isolated under reducing conditions, were used to immunize mice, rabbits and the squirrel monkey Saimiri sciureus. Five or seven subcutaneous injections of each antigenic preparation, in conjunction with Freund's complete or incomplete adjuvants, were administered. This led to the development of specific antibodies detected by IFAT, ELISA or immunoblotting which inhibited merozoite reinvasion in in vitro P. falciparum cultures. This activity seems to be associated with rhoptry proteins contained in fractions Pf F2 and Pf F4.
ISSN:0074-0276
1678-8060
0074-0276
1678-8060
DOI:10.1590/S0074-02761992000700072