Immunogenicity evaluation of recombinant Lactobacillus casei W56 expressing bovine viral diarrhea virus E2 protein in conjunction with cholera toxin B subunit as an adjuvant

• Published in: Microbial cell factories 2020-10, Vol.19 (1), p.186-20, Article 186
• Main Authors: Jia, Shuo, Huang, Xinning, Li, Hua, Zheng, Dianzhong, Wang, Li, Qiao, Xinyuan, Jiang, Yanping, Cui, Wen, Tang, Lijie, Li, Yijing, Xu, Yigang
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic; Administration, Oral; Animals; Antibodies; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; Antibody Formation - immunology; Antigens; Bacteria; Bcl-6 protein; Beef cattle; Bovine viral diarrhea virus (BVDV); Bovine Virus Diarrhea-Mucosal Disease - pathology; Bovine Virus Diarrhea-Mucosal Disease - prevention & control; Cattle; Cattle industry; Cell differentiation; Cell proliferation; Cholera; Cholera toxin; Cholera Toxin - immunology; Cholera toxin B subunit; Cholera toxin B subunit (ctxB); Communicable diseases; Cytokines; Cytokines - immunology; Dendritic cells; Diarrhea; Diarrhea Viruses, Bovine Viral - immunology; Drug delivery systems; E2 protein; Gastrointestinal Tract - pathology; Gastrointestinal Tract - virology; Genetic engineering; Genetically modified organisms; Helper cells; Immune response (cell-mediated); Immune response (humoral); Immunity, Cellular; Immunogenicity; Immunoglobulin A; Infection; Infections; Infectious diseases; Interleukin 10; Interleukin 12; Interleukin 17; Interleukin 2; Interleukin 4; Laboratories; Lacticaseibacillus casei - genetics; Lacticaseibacillus casei - immunology; Lactobacilli; Lactobacillus; Lactobacillus casei; Lymphocytes; Lymphocytes B; Lymphocytes T; Medical research; Mice; Mice, Inbred BALB C; Neutralizing; Pathogens; Plasmids; Proteins; Public health; Recombinant Fusion Proteins - immunology; Recombinant lactobacillus vaccine; Specific Pathogen-Free Organisms; Toxins; Vaccination; Vaccine development; Vaccines; Vaccines, Synthetic - immunology; Viral Envelope Proteins - immunology; Viral Load; Viral Vaccines - immunology; Viruses; Waterborne diseases; γ-Interferon
• ISSN: 1475-2859; 1475-2859
• DOI: 10.1186/s12934-020-01449-3

Bovine viral diarrhea virus (BVDV) is one of the main causes of infectious diseases in cattle and causes large financial losses to the cattle industry worldwide. In this study, Lactobacillus casei strain W56 (Lc W56) was used as antigen deliver carrier to construct a recombinant Lactobacillus vaccine pPG-E2-ctxB/Lc W56 constitutively expressing BVDV E2 protein fused with cholera toxin B subunit (ctxB) as an adjuvant, and its immunogenicity against BVDV infection in mice model by oral route was explored. Our results suggested that pPG-E2-ctxB/Lc W56 can effectively activate dendritic cells (DCs) in the Peyer's patches, up-regulate the expression of Bcl-6, and promote T-follicular helper (Tfh) cells differentiation, as well as enhance B lymphocyte proliferation and promote them differentiate into specific IgA-secreting plasma cells, secreting anti-E2 mucosal sIgA antibody with BVDV-neutralizing activity. Moreover, significant levels (p