Untangling the Defiant Taxonomy of Physaloptera (Nematoda: Chromadorea: Spirurida: Physalopteridae) Parasites in Reptiles: An Integrative Approach on the Enigmatic P. retusa Suggests Cryptic Speciation

• Published in: Taxonomy (Basel, Switzerland) Switzerland), 2024-06, Vol.4 (2), p.326-340
• Main Authors: Ailán-Choke, Lorena Gisela, Ferreira, Vanda Lúcia, Paiva, Fernando, Tavares, Luiz Eduardo Roland, Paschoal, Fabiano, Pereira, Felipe Bisaggio
• Format: Article
• Language: English
• Subjects: Genetic engineering; lizard; Mitochondrial DNA; Morphology; Nematodes; Neotropical Region; Parasites; Phylogenetics; phylogeny; snake; Taxonomy
• ISSN: 2673-6500; 2673-6500
• DOI: 10.3390/taxonomy4020016

(1) Background: Although Physaloptera retusa is one of the most widespread species infecting reptiles in the Americas, numerous taxonomic problems and little genetic data are associated with it. To clarify the taxonomy of this species, we used an integrative approach. (2) Methods: Physaloptera retusa-infecting Erythrolamprus typhlus (snake) from the Pantanal wetlands, Brazil, was morphologically and genetically characterised (18S and 28S rDNA; COI mtDNA) and compared with conspecific sequences available in GenBank, from parasites of Tupinambis teguixin (lizard), using species delimitation methods. Type specimens of P. liophis were re-evaluated given its morphological similarities with P. retusa. (3) Results: The morphology of the present specimens was equal to that of P. retusa, in which the only difference from P. liophis was the relative position of the vulva. Species delimitation methods were more accurate for the COI dataset; all of them (except ABGD) indicated interspecificity among P. retusa sequences. However, a lack of morphological data or voucher material, associated with the deposited sequences, prevented more assertive conclusions. (4) Conclusions: The present results highlight the importance of a clear association between genetic data and morphology of the isolation source, or at least its adequate vouchering. Moreover, P. retusa may represent a species complex in cryptic speciation, since it is widespread and has low hosts specificity.