OPTIMIZATION OF INULINASE PRODUCTION USING COPRA WASTE BY Kluyveromyces marxianus var. marxianus

Kluyveromyces marxianus var. marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase pro¬duction using copra waste as a carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen n...

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Bibliographic Details
Published in:Chemical Industry and Chemical Engineering Quarterly 2010-12, Vol.16 (4), p.319-327
Main Authors: M. DILIPKUMAR, M. RAJASIMMAN, N. RAJAMOHAN
Format: Article
Language:English
Subjects:
copra waste
inulinase
K. marxianus var. marxianus
optimization
RSM
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Summary:Kluyveromyces marxianus var. marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase pro¬duction using copra waste as a carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nut¬rients for their influence on inulinase production was achieved using a Plackett––Burman design. Corn steep liquor, (NH4)2SO4, ZnSO47H2O, K2HPO4 and urea were selected based on their positive influence on inulinase production. The selected components were optimized using response surface methodology (RSM). The optimum conditions are: corn steep liquor – 0.0560 (g/gds), (NH4)2SO4 – 0.0084 (g/gds), ZnSO47H2O – 0.0254 (g/gds), K2HPO4 – 0.0037 (g/gds) and urea - 0.02147 (g/gds). These conditions were validated experimentally which revealed an enhanced inulinase yield of 372 U/gds.
ISSN:1451-9372
DOI:10.2298/CICEQ091211032D