Liver microsystems in vitro for drug response

Engineering approaches were adopted for liver microsystems to recapitulate cell arrangements and culture microenvironments in vivo for sensitive, high-throughput and biomimetic drug screening. This review introduces liver microsystems in vitro for drug hepatotoxicity, drug-drug interactions, metabol...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical science 2019-10, Vol.26 (1), p.88-10, Article 88
Main Authors: Lee, Jyong-Huei, Ho, Kuan-Lun, Fan, Shih-Kang
Format: Article
Language:English
Subjects:
Analysis
Artificial liver
Bioengineering
Cell Engineering - methods
Cell micropatterning
Design and construction
Dose-response relationship
Drug Evaluation, Preclinical - instrumentation
Drug Evaluation, Preclinical - methods
Drug response
Engineered liver microsystems
Humans
Hydrogel biofabrication
In vitro tests
Liver
Methods
Microfluidic perfusion
Microtechnology - instrumentation
Pharmaceutical Preparations - metabolism
Review
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Engineering approaches were adopted for liver microsystems to recapitulate cell arrangements and culture microenvironments in vivo for sensitive, high-throughput and biomimetic drug screening. This review introduces liver microsystems in vitro for drug hepatotoxicity, drug-drug interactions, metabolic function and enzyme induction, based on cell micropatterning, hydrogel biofabrication and microfluidic perfusion. The engineered microsystems provide varied microenvironments for cell culture that feature cell coculture with non-parenchymal cells, in a heterogeneous extracellular matrix and under controllable perfusion. The engineering methods described include cell micropatterning with soft lithography and dielectrophoresis, hydrogel biofabrication with photolithography, micromolding and 3D bioprinting, and microfluidic perfusion with endothelial-like structures and gradient generators. We discuss the major challenges and trends of liver microsystems to study drug response in vitro.
ISSN:1423-0127
1021-7770
1423-0127
DOI:10.1186/s12929-019-0575-0