Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine

Burkholderia pseudomallei is a Gram-negative, facultative intracellular bacillus that causes the disease melioidosis. B. pseudomallei expresses a number of proteins that contribute to its intracellular survival in the mammalian host. We previously demonstrated that immunization with OMVs derived fro...

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Bibliographic Details
Published in:npj vaccines 2021-01, Vol.6 (1), p.18-10, Article 18
Main Authors: Baker, Sarah M., Settles, Erik W., Davitt, Christopher, Gellings, Patrick, Kikendall, Nicole, Hoffmann, Joseph, Wang, Yihui, Bitoun, Jacob, Lodrigue, Kasi-Russell, Sahl, Jason W., Keim, Paul, Roy, Chad, McLachlan, James, Morici, Lisa A.
Format: Article
Language:English
Subjects:
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Bacterial infections
Biomedical and Life Sciences
Biomedicine
Gram-negative bacteria
Growth conditions
Immunization
Infectious Diseases
Medical Microbiology
Proteins
Public Health
Vaccine
Vaccines
Virology
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Summary:Burkholderia pseudomallei is a Gram-negative, facultative intracellular bacillus that causes the disease melioidosis. B. pseudomallei expresses a number of proteins that contribute to its intracellular survival in the mammalian host. We previously demonstrated that immunization with OMVs derived from B. pseudomallei grown in nutrient-rich media protects mice against lethal disease. Here, we evaluated if OMVs derived from B. pseudomallei grown under macrophage-mimicking growth conditions could be enriched with intracellular-stage proteins in order to improve the vaccine. We show that OMVs produced in this manner (M9 OMVs) contain proteins associated with intracellular survival yet are non-toxic to living cells. Immunization of mice provides significant protection against pulmonary infection similar to that achieved with a live attenuated vaccine and is associated with increased IgG, CD4 + , and CD8 + T cells. OMVs possess inherent adjuvanticity and drive DC activation and maturation. These results indicate that M9 OMVs constitute a new promising vaccine against melioidosis.
ISSN:2059-0105
2059-0105
DOI:10.1038/s41541-021-00281-z