Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method

The Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tissue...

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Published in:TheScientificWorld 2014-01, Vol.2014 (2014), p.1-6
Main Authors: Murugaiyah, Vikneswaran, Sahgal, Geethaa, Sundarasekar, Jeevandran, Sreeramanan, Subramaniam
Format: Article
Language:English
Subjects:
2,4-Dichlorophenoxyacetic Acid - pharmacology
Amaryllidaceae Alkaloids - analysis
Analytical methods
Benzyl Compounds
Biosynthesis
Chromatography, High Pressure Liquid - methods
Growth regulators
Herbal medicine
High performance liquid chromatography
Kinetin - pharmacology
Liliopsida
Liquid chromatography
Medicinal plants
Metabolites
Methods
Phenanthridines - analysis
Physiological aspects
Plant growth
Plant Growth Regulators - pharmacology
Plants, Medicinal - chemistry
Plants, Medicinal - drug effects
Purines
Studies
Ultraviolet radiation
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Summary:The Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tissue culture samples of H. littoralis. The separation was achieved using a reversed-phase column. The method was found to be accurate, repeatable, and sensitive for the quantification of minute amount of lycorine present in the samples. The highest lycorine content was found in the bulb extract ( 2.54 ± 0.02 μg/mg) whereas the least was in the root extract ( 0.71 ± 0.02 μg/mg) of the wild plants. Few callus culture samples had high content of lycorine, comparable to that of wild plants. The results showed that plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) alone at 4.5 μM ( 2.58 ± 0.38 μg/mg) or a combination of 2,4-D at 9.00 μM with 4.5 μM of 6-benzylaminopurine (BAP), were the optimum concentrations for the production of high lycorine ( 2.45 ± 0.15 μg/mg) content in callus culture. The present analytical method could be of value for routine quantification of lycorine in the tissue culture production and standardization of the raw material or extracts of H. littoralis.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2014/408306