Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator Arrays

Resonant glassy nanostrings have been employed for the detection of biomolecules. These devices offer high sensitivity and amenability to large array integration and multiplexed assays. Such a concept has however been impaired by the lack of stable and biocompatible linker chemistries. Diazonium sal...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2015-07, Vol.15 (8), p.18724-18741
Main Authors: Zheng, Wei, Du, Rongbing, Cao, Yong, Mohammad, Mohammad A, Dew, Steven K, McDermott, Mark T, Evoy, Stephane
Format: Article
Language:English
Subjects:
Animals
Biosensing Techniques - instrumentation
biosensors
Diazonium Compounds - chemistry
Glass - chemistry
Helium
Immunoglobulin G - chemistry
interferometry
Ions
Molecular Probes - chemistry
nanomechanics
Nanoparticles - chemistry
Nanoparticles - ultrastructure
nanostrings
Photoelectron Spectroscopy
Rabbits
Silicon Compounds - chemistry
Surface Properties
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Summary:Resonant glassy nanostrings have been employed for the detection of biomolecules. These devices offer high sensitivity and amenability to large array integration and multiplexed assays. Such a concept has however been impaired by the lack of stable and biocompatible linker chemistries. Diazonium salt reduction-induced aryl grafting is an aqueous-based process providing strong chemical adhesion. In this work, diazonium-based linker chemistry was performed for the first time on glassy nanostrings, which enabled the bio-functionalization of such devices. Large arrays of nanostrings with ultra-narrow widths down to 10 nm were fabricated employing electron beam lithography. Diazonium modification was first developed on SiCN surfaces and validated by X-ray photoelectron spectroscopy. Similarly modified nanostrings were then covalently functionalized with anti-rabbit IgG as a molecular probe. Specific enumeration of rabbit IgG was successfully performed through observation of downshifts of resonant frequencies. The specificity of this enumeration was confirmed through proper negative control experiments. Helium ion microscopy further verified the successful functionalization of nanostrings.
ISSN:1424-8220
1424-8220
DOI:10.3390/s150818724