Potential of 2-Chloro- N -(4-fluoro-3-nitrophenyl)acetamide Against Klebsiella pneumoniae and In Vitro Toxicity Analysis

• Published in: Molecules (Basel, Switzerland) Switzerland), 2020-08, Vol.25 (17), p.3959
• Main Authors: Cordeiro, Laísa, Diniz-Neto, Hermes, Figueiredo, Pedro, Souza, Helivaldo, Sousa, Aleson, Andrade-Júnior, Francisco, Melo, Thamara, Ferreira, Elba, Oliveira, Rafael, Athayde-Filho, Petrônio, Barbosa-Filho, José, Oliveira-Filho, Abrahão, Lima, Edeltrudes
• Format: Article
• Language: English
• Subjects: 2-chloro-N-(4-fluoro-3-nitrophenyl)acetamide; Abscesses; Acetamides - chemical synthesis; Acetamides - chemistry; Acetamides - pharmacology; Acetamides - toxicity; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - toxicity; antibacterial; Antibacterial activity; Antibiotics; Antimicrobial agents; Bacteria; Biocompatibility; Biological activity; Carbon; Chemistry Techniques, Synthetic; Cytotoxicity; Drug resistance; Gram-negative bacteria; Hemolysis - drug effects; Humans; In vivo methods and tests; Klebsiella; Klebsiella Infections - drug therapy; Klebsiella Infections - microbiology; Klebsiella pneumoniae; Klebsiella pneumoniae - drug effects; Lysis; Microbial Sensitivity Tests; minimum inhibitory concentration; Models, Molecular; Molecular Conformation; Molecular Structure; Mutagenicity; NMR; Nosocomial infection; Nosocomial infections; Nuclear magnetic resonance; Pathogens; Penicillin; Penicillin-binding protein; Pharmacokinetics; Pharmacology; Pneumonia; Structure-Activity Relationship; toxicity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25173959

causes a wide range of community and nosocomial infections. The high capacity of this pathogen to acquire resistance drugs makes it necessary to develop therapeutic alternatives, discovering new antibacterial molecules. Acetamides are molecules that have several biological activities. However, there are no reports on the activity of 2-chloro- -(4-fluoro-3-nitrophenyl)acetamide. Based on this, this study aimed to investigate the in vitro antibacterial activity of this molecule on , evaluating whether the presence of the chloro atom improves this effect. Then, analyzing its antibacterial action more thoroughly, as well as its cytotoxic and pharmacokinetic profile, in order to contribute to future studies for the viability of a new antibacterial drug. It was shown that the substance has good potential against and the chloro atom is responsible for improving this activity, stabilizing the molecule in the target enzyme at the site. The substance possibly acts on penicillin-binding protein, promoting cell lysis. The analysis of cytotoxicity and mutagenicity shows favorable results for future in vivo toxicological tests to be carried out, with the aim of investigating the potential of this molecule. In addition, the substance showed an excellent pharmacokinetic profile, indicating good parameters for oral use.