Whole conversion of agro-industrial wastes rich in galactose-based carbohydrates into lipid using oleaginous yeast Aureobasidium namibiae

• Published in: Biotechnology for biofuels 2021-09, Vol.14 (1), p.1-181, Article 181
• Main Authors: Wang, Zhi-Peng, Zhang, Xin-Yue, Ma, Yan, Ye, Jing-Run, Jiang, Jing, Wang, Hai-Ying, Chen, Wei
• Format: Article
• Language: English
• Subjects: Agricultural wastes; Aureobasidium; Biomass; Carbohydrates; Carbon; Carbon sources; Fatty acids; Galactose; Galactosidase; Glucose; Hydrolysis; Industrial wastes; Lactose; Lipid; Lipids; Methods; Microorganisms; Molasses; Monosaccharides; Production processes; Raffinose; Raw materials; Soy molasses; Stachyose; Sucrose; Sugarcane; Syrups & sweeteners; Transcription; Whey; Whey powder; Yeast; Yeast fungi; Yeasts; α-Galactosidase; β-Galactosidase
• ISSN: 1754-6834; 1754-6834
• DOI: 10.1186/s13068-021-02031-8

Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C.sub.16:0, C.sub.18:0, C.sub.18:1, and C.sub.18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three [alpha]-galactosidases and five [beta]-galactosidases in the strain were cloned and analyzed. Changes of transcriptional levels indicated GalB and GalC were key [alpha]-galactosidases, and GalG was key [beta]-galactosidase. In 10 L fermentor, lipid production from SM and WP achieved 6.45 g/L and 6.13 g/L, respectively. [beta]-galactosidase was responsible for lactose hydrolysis; sucrase and [alpha]-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. This is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess.