In vitro-induction of metronidazole-resistant Giardia duodenalis is not associated with nucleotide alterations in the genes involved in pro-drug activation

Giardiasis is an infectious disease caused by Giardia duodenalis . The pro-drug metronidazole (MTZ) is the first-line treatment for giardiasis. Parasite’s proteins as pyruvate:ferredoxin oxidoreductase (PFOR), ferredoxin (Fd), nitroreductase-1 (NR-1) and thioredoxin reductase (TrxR) participate in M...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2020-01, Vol.115, p.e200303-e200303
Main Authors: Lopes-Oliveira, Luiz Antonio Pimentel, Fantinatti, Maria, Da-Cruz, Alda Maria
Format: Article
Language:English
Subjects:
Giardia duodenalis
metronidazole
PARASITOLOGY
resistance
Short Communication
TROPICAL MEDICINE
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Summary:Giardiasis is an infectious disease caused by Giardia duodenalis . The pro-drug metronidazole (MTZ) is the first-line treatment for giardiasis. Parasite’s proteins as pyruvate:ferredoxin oxidoreductase (PFOR), ferredoxin (Fd), nitroreductase-1 (NR-1) and thioredoxin reductase (TrxR) participate in MTZ activation. Here, we showed Giardia trophozoites long-term exposed to MTZ presented higher IC 50 than controls, showing the drug influenced the parasite survival. That reduction in MTZ’s susceptibility does not seem to be related to mutations in the genes pfor , fd , nr-1 or trxr . It points that different mechanism as alterations in other metabolic pathways can account for Giardia resistance to MTZ therapy.
ISSN:0074-0276
1678-8060
1678-8060
DOI:10.1590/0074-02760200303