Decreased placental and muscular expression of the fibroblast growth factor 19 in gestational diabetes mellitus

• Published in: Journal of diabetes investigation 2019-01, Vol.10 (1), p.171-181
• Main Authors: Wang, Dongyu, Xu, Shuqia, Ding, Wenjing, Zhu, Caixia, Deng, Songqing, Qiu, Xiwen, Wang, Zilian
• Format: Article
• Language: English
• Subjects: Adult; Animal models; Diabetes; Diabetes mellitus; Diabetes, Gestational - metabolism; Female; Fibroblast growth factor 19; Fibroblast growth factors; Fibroblast Growth Factors - metabolism; Fibroblasts; Gene expression; Gestational diabetes mellitus; Growth factors; Homeostasis; Humans; Immunohistochemistry; Insulin resistance; Klotho protein; Klotho Proteins; Lipid metabolism; Membrane Proteins - metabolism; mRNA; Original; Placenta; Placenta - metabolism; Polymerase chain reaction; Pregnancy; Proteins; Rectus Abdominis - metabolism; Rectus muscle; RNA, Messenger; Subcutaneous Fat
• ISSN: 2040-1116; 2040-1124
• DOI: 10.1111/jdi.12859

Aims/Introduction Fibroblast growth factor (FGF)19 has been shown to improve glycemic homeostasis and lipid metabolism in animal models. In humans, decreased FGF19 level has been described in diabetes. The present study aimed to investigate the expression of FGF19 in gestational diabetes mellitus (GDM) patients. Materials and Methods Samples for measurement were obtained from 20 women with GDM and 25 healthy controls. The messenger ribonucleic acid (mRNA) and protein expression levels of FGF19, FGF21 and co‐receptor β‐klotho (KLB) in the placenta, rectus muscle and subcutaneous fat tissues were quantified by real‐time quantitative polymerase chain reaction, western blot and immunohistochemistry, respectively. Results Women with GDM had significantly lower mRNA and protein expressions of FGF19 than control women in the placenta (mRNA 0.33 ± 0.05 vs 0.72 ± 0.09; protein 0.34 ± 0.13 vs 0.85 ± 0.20) and rectus muscle (mRNA 0.83 ± 0.11 vs 1.28 ± 0.19; protein 0.78 ± 0.24 vs 1.23 ± 0.39). However, there were no significant differences between GDM women and controls with respect to the expression levels of FGF21 and β‐klotho in the placenta and rectus muscle. There were almost no detectable FGF19 and FGF21 expressions in subcutaneous fat tissue. Furthermore, β‐klotho expression levels were not different between the GDM and control group in subcutaneous fat. Conclusions FGF19 expressions are decreased in the placenta and rectus muscle of women with GDM. This might contribute to the pathophysiology or development of GDM. FGF19 expressions are decreased in GDM women's placenta and rectus muscle. This may contribute to the pathophysiology or development of GDM.