Phytochemical Composition and Antimicrobial and Antibiofilm Effect of Myrciaria cauliflora Hydroethanolic Extract against Staphylococcus aureus and Acinetobacter baumannii

• Published in: Methods and protocols 2024-08, Vol.7 (4), p.60
• Main Authors: de Oliveira, Luciane Dias, Ribeiro, Ana Luisa Monteiro, Dias, Sthéfani de Oliveira, da Cruz, Geovani Moreira, de Menezes, Raquel Teles, de Carvalho, Lara Steffany, Diamantino, Mariana Gadelho Gimenez, Pereira, Thaís Cristine, Marcucci, Maria Cristina, Abu Hasna, Amjad
• Format: Article
• Language: English
• Subjects: Acinetobacter baumannii; Antibiotics; antimicrobial and antibiofilm effect; Bacteria; Biofilms; Catheters; Chemical composition; Chromatography; Cinnamic acid; Ellagic acid; Flavonoids; Gallic acid; herbal extracts; Herbal medicine; High-performance liquid chromatography; Immune system; Intensive care; Medical equipment; Microorganisms; Minimum inhibitory concentration; Myrciaria cauliflora; Nosocomial infections; Opportunist infection; p-Coumaric acid; Pathogens; Phenols; phytochemical composition; Phytochemicals; Quercetin; Solvents; Staphylococcus aureus; Staphylococcus infections; Statistical analysis; Strains (organisms)
• ISSN: 2409-9279; 2409-9279
• DOI: 10.3390/mps7040060

and are opportunistic pathogens, and both are involved in different oral infections. This work aimed to analyze the phytochemical composition of hydroethanolic extract and to evaluate its antimicrobial and antibiofilm action against (ATCC 6538) and (ATCC 19606; multi-resistant clinical strains 58004, 50098, 566006, and H557). hydroethanolic extract was prepared, and the content of soluble solids, flavonoids, and phenols was quantified. High-performance liquid chromatography (HPLC) was performed later. The minimum inhibitory concentration was determined using the broth microdilution method according to the Clinical and Laboratory Standards Institute, standard M7-A6, and subsequently, its minimum bactericidal concentration was determined. Then, the most effective concentrations were analyzed against biofilms. Statistical analysis was performed using the ANOVA method with Tukey's test. The soluble solids content in the prepared hydroethanolic extract of was 2.22%. Additionally, the total flavonoid content, measured using the quercetin standard curve, was 0.040 mg/mL. Furthermore, the total phenol content, determined using the gallic acid standard curve, was 0.729 mg/mL. HPLC analysis presented peaks of gallic acid (11.80 m), p-coumaric acid (12.09 m), cinnamic acid derivative (19.02 m), and ellagic acid (29.83 m). The extract demonstrated antimicrobial and antibiofilm action against all tested strains. However, the most effective antibacterial concentration against all the tested bacteria was 5.55 mg/mL. Therefore, these chemical components justify that hydroethanolic extract is effective in reducing biofilm formation in (standard strain) and (standard and clinical strains).