Comparison between colorimetric in Situ hybridization, histopathology, and immunohistochemistry for the diagnosis of new world cutaneous leishmaniasis in human skin samples

New world cutaneous leishmaniasis (NWCL) is an anthropozoonosis caused by different species of the protozoan 'Leishmania'. Colorimetric in situ hybridization (CISH) was shown to satisfactorily detect amastigote forms of 'Leishmania' spp. in animal tissues, yet it was not tested f...

Full description

Saved in:
Bibliographic Details
Published in:Tropical medicine and infectious disease 2022-11, Vol.7 (11), p.1-13
Main Authors: Ferreira, Luiz Cláudio, Quintella, Leonardo Pereira, Schubach, Armando de Oliveira, Miranda, Luciana de Freitas Campos, Madeira, Maria de Fátima, Pimentel, Maria Inês Fernandes, Vasconcellos, Érica de Camargo Ferreira e, Lyra, Marcelo Rosandiski, Oliveira, Raquel de Vasconcellos Carvalhaes de, Menezes, Rodrigo Caldas
Format: Article
Language:English
Subjects:
Antigens
Biopsy
Care
Diagnosis
Enzymes
Ethanol
Histopathology
Hybridization
Immunohistochemistry
Immunological aspects
immunoperoxidase
in situ hybridization
Infectious diseases
Laboratories
Leishmania
Leishmania braziliensis
Parasites
Parasitic diseases
Patients
Performance evaluation
Technique
tegumentary leishmaniasis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:New world cutaneous leishmaniasis (NWCL) is an anthropozoonosis caused by different species of the protozoan 'Leishmania'. Colorimetric in situ hybridization (CISH) was shown to satisfactorily detect amastigote forms of 'Leishmania' spp. in animal tissues, yet it was not tested for the diagnosis of human NWCL. The aim of this study was to compare CISH, histopathology (HP), and immunohistochemistry (IHC) techniques to diagnose NWCL in human cutaneous lesions. The sample comprised fifty formalin-fixed, paraffin-embedded skin biopsy specimens from patients with NWCL caused by 'L. (V.) braziliensis'. These specimens were analyzed by CISH, using a generic probe for 'Leishmania', IHC, and HP to assess the sensitivity of these methods by using a parasitological culture as a standard reference. Additional specimens from three patients diagnosed with cutaneous mycoses were also included to evaluate cross-reactions between CISH and IHC. The sensitivities of IHC, CISH, and HP for detecting amastigotes was 66%, 54%, and 50%, respectively. IHC, unlike CISH, cross-reacted with different species of fungi. Together, these results demonstrate that CISH may be a complementary assay for the detection of amastigote in the laboratorial diagnosis routine of human NWCL caused by 'L. (V.) braziliensis'.
ISSN:2414-6366
2414-6366
DOI:10.3390/tropicalmed7110344