Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench)

A novel type of aspartic proteinase gene was isolated from the cDNA library of developing buckwheat seeds. This cDNA, FeAPL1, encoded an AP-like protein lacking the plant-specific insert (PSI) domain characteristic of typical plant aspartic proteinases. In addition the corresponding genomic fragment...

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Bibliographic Details
Published in:Archives of biological sciences 2007, Vol.59 (2), p.119-124
Main Authors: Milisavljevic, M.Dj.,Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia), Timotijevic, G.S.,Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia), Radovic, S.R.,University of Belgrade, Belgrade (Serbia). Faculty of Biology, Konstantinovic, M.M.,Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia), Maksimovic, V.R.,Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia)
Format: Article
Language:English
Subjects:
ADN
aspartic proteinase
buckwheat
cDNA
DNA
FAGOPYRUM ESCULENTUM
GENE
GENES
http://www.fao.org/aos/agrovoc#c_2347
http://www.fao.org/aos/agrovoc#c_2777
http://www.fao.org/aos/agrovoc#c_3214
http://www.fao.org/aos/agrovoc#c_6242
PROTEASAS
PROTEASE
PROTEASES
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Summary:A novel type of aspartic proteinase gene was isolated from the cDNA library of developing buckwheat seeds. This cDNA, FeAPL1, encoded an AP-like protein lacking the plant-specific insert (PSI) domain characteristic of typical plant aspartic proteinases. In addition the corresponding genomic fragment was isolated. It is demonstrated that this gene does not contain introns. Since bioinformatics analysis of the Arabidopsis genomes showed that most potential AP genes are intron-less and PSI-less, it appears that atypical is an inappropriate work for that class of AP. Isolation of this specific buckwheat gene among the small group of those isolated from other plant species provides a new perspective on the diversity of AP family members in plants.
ISSN:0354-4664
1821-4339
DOI:10.2298/ABS0702119M