Distinct mode of action of a highly stable, engineered phage lysin killing Gram-negative bacteria

Engineered lysins are considered as highly promising alternatives for antibiotics. Our previous screening study using VersaTile technology identified 1D10 as a possible lead compound with activity against strains under elevated human serum concentrations. In this manuscript, we reveal an unexpected...

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Bibliographic Details
Published in:Microbiology spectrum 2023-12, Vol.11 (6), p.e0181323-e0181323
Main Authors: Gerstmans, Hans, Duyvejonck, Lisa, Vázquez, Roberto, Staes, Ines, Borloo, Jimmy, Abdelkader, Karim, Leroy, Jeroen, Cremelie, Emma, Gutiérrez, Diana, Tamés-Caunedo, Héctor, Ruas-Madiedo, Patricia, Rodríguez, Ana, Aertsen, Abram, Lammertyn, Jeroen, Lavigne, Rob, Briers, Yves
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Bacteriophages
lysin
mode of action
osmotic lysis
Research Article
serum
thermoresistance
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Summary:Engineered lysins are considered as highly promising alternatives for antibiotics. Our previous screening study using VersaTile technology identified 1D10 as a possible lead compound with activity against strains under elevated human serum concentrations. In this manuscript, we reveal an unexpected mode of action and exceptional thermoresistance for lysin 1D10. Our findings shed new light on the development of engineered lysins, providing valuable insights for future research in this field.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.01813-23