Comparison of growth factor levels in injectable platelet-rich fibrin obtained from healthy individuals and patients with chronic periodontitis: a pilot study

• Published in: BMC oral health 2024-05, Vol.24 (1), p.527-527, Article 527
• Main Authors: Karcı, Bilge, Savas, Hasan Basri
• Format: Article
• Language: English
• Subjects: Adult; Anticoagulants; Becaplermin - blood; Blood cell count; Blood platelets; Blood Platelets - metabolism; Blood tests; Case-Control Studies; Chronic periodontitis; Chronic Periodontitis - blood; Comparative analysis; Dental research; Enzyme-linked immunosorbent assay; Epidermal growth factor; Epidermal Growth Factor - analysis; Epidermal Growth Factor - blood; Ethylenediaminetetraacetic acid; Female; Fibrin; Growth factors; Gum disease; Health aspects; Humans; Injectable platelet rich fibrin; Injections; Insulin-like growth factor I; Insulin-Like Growth Factor I - analysis; Insulin-like growth factors; Intercellular Signaling Peptides and Proteins - analysis; Intercellular Signaling Peptides and Proteins - blood; Leukocyte Count; Leukocytes; Male; Maxillofacial surgery; Medical research; Medicine, Experimental; Middle Aged; Patients; Periodontal diseases; Periodontics; Periodontitis; Physiological aspects; Pilot Projects; Platelet-derived growth factor; Platelet-derived growth factor BB; Platelet-Rich Fibrin; Platelets; Statistical analysis; Systemic diseases; Transforming Growth Factor beta1 - blood; Transforming growth factor-b1; University faculty; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - blood; White blood cells; Wound healing
• ISSN: 1472-6831; 1472-6831
• DOI: 10.1186/s12903-024-04301-x

This study aimed to assess and compare the concentrations of growth factors, white blood cells (WBCs), and platelets in injectable platelet-rich fibrin (i-PRF) derived from people with healthy periodontal conditions and those with chronic periodontitis. Venous blood samples were obtained from 30 patients diagnosed with chronic periodontitis (test group) and 30 participants with healthy periodontal conditions (control group). The i-PRF was then acquired from centrifuged blood. The growth factors (VEGF, IGF-1, TGF-β1, PDGF-BB and EGF) released from the i-PRF samples were compared between groups with ELISA testing. The amounts of WBCs and platelets were also compared. No significant differences in the concentrations of growth factors were found between the groups (the mean values for the control and test groups were, respectively: IGF: 38.82, 42.46; PDGF: 414.25, 466.28; VEGF: 375.69, 412.18; TGF-β1: 21.50, 26.21; EGF: 138.62, 154.82). The test group exhibited a significantly higher WBC count than the control group (8.80 vs. 6.60, respectively). However, the platelet count did not show a statistically significant difference between the groups (control group 242.0 vs. test group 262.50). No significant correlation was observed between WBC count and growth factor level in either group. The growth factor levels in i-PRFs did not exhibit significant difference between the two groups. This suggests that the levels of these growth factors may be unaffected by the periodontal disease.