Reverse vaccinology and subtractive genomics approaches for identifying common therapeutics against Mycobacterium leprae and Mycobacterium lepromatosis

and are gram-positive bacterial pathogens and the causative agents of leprosy in humans across the world. The elimination of leprosy cannot be achieved by multidrug therapy alone, and highlights the need for new tools and drugs to prevent the emergence of new resistant strains. In this study, our co...

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Published in:The journal of venomous animals and toxins including tropical diseases 2021-04, Vol.27, p.e20200027-e20200027
Main Authors: Jaiswal, Arun Kumar, Tiwari, Sandeep, Jamal, Syed Babar, Oliveira, Letícia de Castro, Sales-Campos, Helioswilton, Andrade-Silva, Leonardo Eurípedes, Oliveira, Carlo Jose Freire, Ghosh, Preetam, Barh, Debmalya, Azevedo, Vasco, Soares, Siomar C, Rodrigues, Virmondes Rodrigues, da Silva, Marcos Vinicius
Format: Article
Language:English
Subjects:
Drug target identification
Leprosy
Mycobacterium leprae
Mycobacterium lepromatosis
TOXICOLOGY
TROPICAL MEDICINE
Vaccine targets
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Summary:and are gram-positive bacterial pathogens and the causative agents of leprosy in humans across the world. The elimination of leprosy cannot be achieved by multidrug therapy alone, and highlights the need for new tools and drugs to prevent the emergence of new resistant strains. In this study, our contribution includes the prediction of vaccine targets and new putative drugs against leprosy, using reverse vaccinology and subtractive genomics. Six strains of and (4 and 2 strains, respectively) were used for comparison taking strain TN as the reference genome. Briefly, we used a combined reverse vaccinology and subtractive genomics approach. As a result, we identified 12 common putative antigenic proteins as vaccine targets and three common drug targets against and Furthermore the docking analysis using 28 natural compounds with three drug targets was done. The bis-naphthoquinone compound Diospyrin (CID 308140) obtained from indigenous plant spp showed the most favored binding affinity against predicted drug targets, which can be a candidate therapeutic target in the future against leprosy.
ISSN:1678-9199
1678-9199
DOI:10.1590/1678-9199-JVATITD-2020-0027