Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells

Background/Aims: Probiotics play a role in relieving irritable bowel syndrome (IBS); however, the underlying mechanism is yet unclear. The aim of the study was to investigate the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transp...

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Published in:Saudi journal of gastroenterology 2018-01, Vol.24 (1), p.59-66
Main Authors: Cao, Ya-Nan, Feng, Li-Juan, Wang, Bang-Mao, Jiang, Kui, Li, Shu, Xu, Xin, Wang, Wei-Qiang, Zhao, Jing-Wen, Wang, Yu-Ming
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Bifidobacteria
Bifidobacterium longum supernatant
Deoxyribonucleic acid
DNA
Gastroenterology
Gene expression
Growth factors
Hepatology
intestinal epithelial cells
Irritable bowel syndrome
Lactobacillus acidophilus
Lactobacillus acidophilus supernatant
Life sciences
Original
Probiotics
Proteins
Rodents
Serotonin
serotonin transporter
Yogurt
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Summary:Background/Aims: Probiotics play a role in relieving irritable bowel syndrome (IBS); however, the underlying mechanism is yet unclear. The aim of the study was to investigate the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein. Materials and Methods: HT-29 and Caco-2 cells were treated with different concentrations of L. acidophilus and B. longum supernatants for 12 h and 24 h, respectively. SERT mRNA and proteins levels were detected by real-time polymerase chain reaction (real-time PCR) and Western-blotting. Results: The mRNA levels of SERT in HT-29 and Caco-2 cells treated with different concentrations of L. acidophilus or B. longum supernatants for 12 h and 24 h, each, were higher than that in the control groups. In addition, the expression of the protein in both cells was also upregulated, which was approximately similar to that of the corresponding mRNA. Conclusions: L. acidophilus and B. longum supernatants can upregulate SERT mRNA and protein levels in intestinal epithelial cells.
ISSN:1319-3767
1998-4049
DOI:10.4103/sjg.sjg_333_17