Effects of Nutrients and Growth Regulators on Seed Germination and Development of Juvenile Rhizome Proliferation of Gastrodia elata In Vitro

Two prevalent issues with Gastrodia elata growing in a natural wild environment are the necessary symbiotic seed germination with Mycena spp. and the long growing cycle of protocorms into mature rhizomes with Armillaria mellea. This study explored the most suitable nutrients to germinate seeds and t...

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Published in:Agriculture (Basel) 2022-08, Vol.12 (8), p.1210
Main Authors: Hsieh, Chi-Hung, Liang, Zeng-Chin, Shieh, Wen-Jang, Chang, Shin-Liang, Ho, Wai-Jane
Format: Article
Language:English
Subjects:
Acetic acid
Alzheimer's disease
Ammonium nitrate
Aquatic plants
Chitosan
Developmental stages
Gastrodia elata
Germination
Glucose
Glutamic acid
Growth regulators
Humic acids
in vitro seed germination
Indoleacetic acid
juvenile rhizome multiplication
Nutrients
organic additives
Ovaries
Peptones
Plant growth
plant growth regulators
Plant reproduction
Potatoes
Propagation
protocorm development
Rhizomes
Salts
Seed germination
Seeds
Sucrose
Tissue culture
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Summary:Two prevalent issues with Gastrodia elata growing in a natural wild environment are the necessary symbiotic seed germination with Mycena spp. and the long growing cycle of protocorms into mature rhizomes with Armillaria mellea. This study explored the most suitable nutrients to germinate seeds and the developmental stages and propagated protocorm/juvenile rhizomes (jrhs) through a tissue culture approach. Adding humic acid, mashed potato, peptone, and (indole-3-acetic acid) IAA to the 2 g/L Hyponex 7-6-19 basal medium accelerated seed germination into protocorms with an average length of 2 mm. The nutrients best for the in vitro propagation of jrhs consisted of 1 g/L Hyponex 7-6-19 and MS salts with 1/2 NH4NO3 and KNO3, 2 mg/L BA, 2 mg/L NAA, 2 g/L tryptone, 2 g/L glutamic acid, 1 g/L chitosan, 10 g/L apple puree, 20 g/L mashed potato, 100 mL/L coconut water, and 1.5 g/L A. mellea powder, at a pH level of 6. The maximum length and number of jrhs were 17.2 ± 0.8 mm and 76.6, respectively, in two subcultures. The in vitro propagation system started from seeds of G. elata can be employed as a potential method to propagate protocorm/jrhs for the cultivation of mature rhizomes from indoor or outdoor cultures.
ISSN:2077-0472
2077-0472
DOI:10.3390/agriculture12081210