Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons

Somatosensory neurons with cell bodies in the dorsal root ganglia (DRG) project to the skin, muscles, bones, and viscera to detect touch and temperature as well as to mediate proprioception and many types of interoception. In addition, the somatosensory system conveys the clinically relevant noxious...

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Bibliographic Details
Published in:eLife 2021-11, Vol.10
Main Authors: Nguyen, Minh Q, von Buchholtz, Lars J, Reker, Ashlie N, Ryba, Nicholas Jp, Davidson, Steve
Format: Article
Language:English
Subjects:
Adult
Animals
Cell Nucleus - genetics
Dorsal root ganglia
Female
Ganglia, Spinal - metabolism
Gene Expression Profiling
Genes
Genomics
Humans
itch
Male
Mice
Middle Aged
Muscles
Neurons
Neurons - metabolism
Neuroscience
Pain
Proprioception
Single-Cell Analysis
somatosensation
Somatosensory system
Species
Tools and Resources
Transcriptome
Transcriptomics
Viscera
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Summary:Somatosensory neurons with cell bodies in the dorsal root ganglia (DRG) project to the skin, muscles, bones, and viscera to detect touch and temperature as well as to mediate proprioception and many types of interoception. In addition, the somatosensory system conveys the clinically relevant noxious sensations of pain and itch. Here, we used single nuclear transcriptomics to characterize transcriptomic classes of human DRG neurons that detect these diverse types of stimuli. Notably, multiple types of human DRG neurons have transcriptomic features that resemble their mouse counterparts although expression of genes considered important for sensory function often differed between species. More unexpectedly, we identified several transcriptomic classes with no clear equivalent in the other species. This dataset should serve as a valuable resource for the community, for example as means of focusing translational efforts on molecules with conserved expression across species.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.71752