Microencapsulation and the Characterization of Polyherbal Formulation (PHF) Rich in Natural Polyphenolic Compounds

• Published in: Nutrients 2018-06, Vol.10 (7), p.843
• Main Authors: Hussain, Syed Ammar, Hameed, Ahsan, Nazir, Yusuf, Naz, Tahira, Wu, Yang, Suleria, Hafiz Ansar Rasul, Song, Yuanda
• Format: Article
• Language: English
• Subjects: Amylases; Antidiabetics; antioxidant activity; Antioxidants; Assaying; Bioactive compounds; Bioavailability; Diabetes mellitus; Encapsulation; Flavonoids; Freeze drying; Gelatin; Glass transition temperature; Glucosidase; Hygroscopicity; in vitro anti-diabetic potential; in vitro dialyzability; Maltodextrin; microencapsulation; Moisture content; Particle size; Phenolic compounds; Phenols; Photochemicals; Polyphenols; Preservation; Solubility; Tannins; Temperature; Transition temperatures; Water activity; Water content; α-Amylase; α-Glucosidase; β-Carotene
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu10070843

Microencapsulation of polyherbal formulation (PHF) extract was carried out by freeze drying method, by employing gum arabic (GA), gelatin (GE), and maltodextrin (MD) with their designated different combinations as encapsulating wall materials. Antioxidant components (i.e., total phenolic contents (TPC), total flavonoids contents (TFC), and total condensed tannins (TCT)), antioxidant activity (i.e., DPPH, β-carotene & ABTS⁺ assays), moisture contents, water activity (a ), solubility, hygroscopicity, glass transition temperature (T ), particle size, morphology, in vitroα-amylase and α-glucosidase inhibition and bioavailability ratios of the powders were investigated. Amongst all encapsulated products, T (5% GA & 5% MD) and T (10% GA) have proven to be the best treatments with respect to the highest preservation of antioxidant components. These treatments also exhibited higher antioxidant potential by DPPH and β-carotene assays and noteworthy for an ABTS⁺ assays. Moreover, the aforesaid treatments also demonstrated lower moisture content, a , particle size and higher solubility, hygroscopicity and glass transition temperature (T ). All freeze dried samples showed irregular (asymmetrical) microcrystalline structures. Furthermore, T and T also illustrated the highest in vitro anti-diabetic potential due to great potency for inhibiting α-amylase and α-glucosidase activities. In the perspective of bioavailability, T , T and T demonstrated the excellent bioavailability ratios (%). Furthermore, the photochemical profiling of ethanolic extract of PHF was also revealed to find out the bioactive compounds.