Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot: Usefulness in Clinical Practice

Anti-TIF-1γ autoantibody detection is important for cancer screening in patients with dermatomyositis. The gold standard for anti-TIF-1γ detection, immunoprecipitation, is only available from a few specialized laboratories worldwide, so commercial ELISA/immunoblot tests have emerged in recent years....

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Published in:Frontiers in immunology 2021-02, Vol.11, p.625896-625896
Main Authors: Mariscal, Anaís, Milán, Milena, Baucells, Andrés, Martínez, Maria Angeles, Guillen, Andrea Garcia, Trallero-Araguás, Ernesto, Alvarado-Cardenas, Marcelo, Martínez-Martínez, Laura, Alserawan, Leticia, Franco-Leyva, Teresa, Sanz-Martínez, María Teresa, Viñas-Giménez, Laura, Corominas, Hector, Juárez, Cándido, Castellví, Iván, Selva-O'Callaghan, Albert
Format: Article
Language:English
Subjects:
autoantibody
cancer
dermatomyositis
diagnosis
immunoassay
Immunology
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Summary:Anti-TIF-1γ autoantibody detection is important for cancer screening in patients with dermatomyositis. The gold standard for anti-TIF-1γ detection, immunoprecipitation, is only available from a few specialized laboratories worldwide, so commercial ELISA/immunoblot tests have emerged in recent years. To analyze their usefulness in diagnosing cancer-associated dermatomyositis, we compared Euroimmun Euroline profile with our previously validated in-house immunoblot assay with human recombinant TIF-1γ. We included 308 adult patients from Hospital de la Santa Creu I Sant Pau and Vall Hebrón Hospital (Barcelona, Spain) tested for anti-TIF-1γ autoantibodies using the Euroline profile and an in-house immunoblot assay. A total of 27 anti-TIF-1γ were detected by the Euroline and 12 by the in-house assay. Fair agreement was observed between Euroline and the in-house immunoblot Cohen's kappa 0.3163. Expected prevalence of anti-TIF-1γ autoantibodies was observed for the two methods for dermatomyositis and undifferentiated connective tissue diseases, but unexpectedly high prevalence of anti-TIF-1γ autoantibodies was detected by Euroline compared to the in-house immunoblot for other diseases (16.5% Euroline vs 0.8% in-house immunoblot, p
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2020.625896