Human Maf1 negatively regulates RNA polymerase III transcription via the TFIIB family members Brf1 and Brf2

RNA polymerase III (RNA pol III) transcribes many of the small structural RNA molecules involved in processing and translation, thereby regulating the growth rate of a cell. Initiation of pol III transcription requires the evolutionarily conserved pol III initiation factor TFIIIB. TFIIIB is the mole...

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Bibliographic Details
Published in:International journal of biological sciences 2007-05, Vol.3 (5), p.292-302
Main Authors: Rollins, Janet, Veras, Ingrid, Cabarcas, Stephanie, Willis, Ian, Schramm, Laura
Format: Article
Language:English
Subjects:
HeLa Cells
Humans
Luciferases, Firefly - genetics
Luciferases, Renilla
Promoter Regions, Genetic
Repressor Proteins - metabolism
Repressor Proteins - physiology
Research Paper
RNA Polymerase III - metabolism
TATA-Binding Protein Associated Factors - metabolism
Transcription Factor TFIIIB - metabolism
Transcription, Genetic
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Summary:RNA polymerase III (RNA pol III) transcribes many of the small structural RNA molecules involved in processing and translation, thereby regulating the growth rate of a cell. Initiation of pol III transcription requires the evolutionarily conserved pol III initiation factor TFIIIB. TFIIIB is the molecular target of regulation by tumor suppressors, including p53, RB and the RB-related pocket proteins. However, our understanding of negative regulation of human TFIIIB-mediated transcription by other proteins is limited. In this study we characterize a RNA pol III luciferase assay and further demonstrate in vivo that a human homolog of yeast Maf1 represses RNA pol III transcription. Additionally, we show that Maf1 repression of RNA pol III transcription occurs via TFIIIB, specifically through the TFIIB family members Brf1 and Brf2.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.3.292