Synthesis of Extracellular L-lysine-α-oxidase along with Degrading Enzymes by Trichoderma cf. aureoviride Rifai VKM F-4268D: Role in Biocontrol and Systemic Plant Resistance

• Published in: Journal of fungi (Basel) 2024-04, Vol.10 (5), p.323
• Main Authors: Arinbasarova, Anna Yu, Botin, Alexander S, Medentsev, Alexander G, Makrushin, Kirill V, Vetcher, Alexandre A, Stanishevskiy, Yaroslav M
• Format: Article
• Language: English
• Subjects: Antimicrobial activity; Antimicrobial agents; Biological control; Biosynthesis; Cancer; Cell death; Cell walls; Cellulase; Chitinase; Deamination; degradative enzymes; Enzymes; Fungi; glucanases; Glucose; Gram-negative bacteria; Gram-positive bacteria; Hydrogen peroxide; Lysine; Metabolites; Organisms; Phytopathogenic fungi; Plant resistance; protease; Trichoderma; Yeast
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof10050323

When cultivating on wheat bran or deactivated fungal mycelium as a model of "natural growth", the ability of to synthesize extracellular L-lysine-α-oxidase (LysO) simultaneously with cell-wall-degrading enzymes (proteases, xylanase, glucanases, chitinases, etc.), responsible for mycoparasitism, was shown. LysO, in turn, causes the formation of H O and pipecolic acid. These compounds are known to be signaling molecules and play an important role in the induction and development of systemic acquired resistance in plants. Antagonistic effects of LysO have been demonstrated against phytopathogenic fungi and Gram-positive or Gram-negative bacteria with dose-dependent cell death. The antimicrobial effect of LysO decreased in the presence of catalase. The generating intracellular ROS in the presence of LysO was also shown in both bacteria and fungi, which led to a decrease in viable cells. These results suggest that the antimicrobial activity of LysO is due to two factors: the formation of exogenous hydrogen peroxide as a product of the enzymatic oxidative deamination of L-lysine and the direct interaction of LysO with the cell wall of the micro-organisms. Thus, LysO on its own enhances the potential of the producer in the environment; namely, the enzyme complements the strategy of the fungus in biocontrol and indirectly participates in inducing SAR and regulating the relationship between pathogens and plants.