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Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil
Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small s...
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Published in: | Parasites & vectors 2017-10, Vol.10 (1), p.518-518, Article 518 |
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description | Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil.
A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers.
Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%).
DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro. |
doi_str_mv | 10.1186/s13071-017-2458-0 |
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A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers.
Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%).
DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro.</description><identifier>ISSN: 1756-3305</identifier><identifier>EISSN: 1756-3305</identifier><identifier>DOI: 10.1186/s13071-017-2458-0</identifier><identifier>PMID: 29070053</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Arthritis ; Blastocystis ; Brazil ; Communities ; Deoxyribonucleic acid ; Developing countries ; digestive tract ; Distribution ; DNA ; DNA sequences ; DNA sequencing ; Entamoeba ; Enterobius vermicularis ; Environmental health ; Epidemiology ; Gastrointestinal system ; Gastrointestinal tract ; genes ; Genetic diversity ; Genetic variation ; Heterokonts ; Human populations ; humans ; Identification ; Infections ; Irritable bowel syndrome ; Laboratories ; LDCs ; Medical research ; Medicine, Experimental ; Nucleic acids ; Nucleotide sequence ; Parasites ; Pavlova ; PCR ; Phylogeny ; Polymerase chain reaction ; Primers ; protists ; Reptiles & amphibians ; Ribonucleic acid ; ribosomal RNA ; RNA ; rRNA ; Sedimentation & deposition ; sequence analysis ; Sequencing ; Studies ; Subtypes ; Taenia ; Trichuris trichiura ; Urban areas ; Zoonoses</subject><ispartof>Parasites & vectors, 2017-10, Vol.10 (1), p.518-518, Article 518</ispartof><rights>COPYRIGHT 2017 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2017</rights><rights>The Author(s). 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-d072075a853bab6c6f52dfafb60f26a05a8424977f7a1cd7613992877f705bf93</citedby><cites>FETCH-LOGICAL-c554t-d072075a853bab6c6f52dfafb60f26a05a8424977f7a1cd7613992877f705bf93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5657060/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1960790513?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29070053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valença Barbosa, Carolina</creatorcontrib><creatorcontrib>de Jesus Batista, Rosemary</creatorcontrib><creatorcontrib>Pereira Igreja, Ricardo</creatorcontrib><creatorcontrib>d'Avila Levy, Claudia Masini</creatorcontrib><creatorcontrib>Werneck de Macedo, Heloisa</creatorcontrib><creatorcontrib>Carneiro Santos, Helena Lúcia</creatorcontrib><title>Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil</title><title>Parasites & vectors</title><addtitle>Parasit Vectors</addtitle><description>Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil.
A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers.
Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%).
DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro.</description><subject>Animals</subject><subject>Arthritis</subject><subject>Blastocystis</subject><subject>Brazil</subject><subject>Communities</subject><subject>Deoxyribonucleic acid</subject><subject>Developing countries</subject><subject>digestive tract</subject><subject>Distribution</subject><subject>DNA</subject><subject>DNA sequences</subject><subject>DNA sequencing</subject><subject>Entamoeba</subject><subject>Enterobius vermicularis</subject><subject>Environmental health</subject><subject>Epidemiology</subject><subject>Gastrointestinal system</subject><subject>Gastrointestinal tract</subject><subject>genes</subject><subject>Genetic diversity</subject><subject>Genetic variation</subject><subject>Heterokonts</subject><subject>Human populations</subject><subject>humans</subject><subject>Identification</subject><subject>Infections</subject><subject>Irritable bowel syndrome</subject><subject>Laboratories</subject><subject>LDCs</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Nucleic acids</subject><subject>Nucleotide sequence</subject><subject>Parasites</subject><subject>Pavlova</subject><subject>PCR</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Primers</subject><subject>protists</subject><subject>Reptiles & amphibians</subject><subject>Ribonucleic acid</subject><subject>ribosomal RNA</subject><subject>RNA</subject><subject>rRNA</subject><subject>Sedimentation & deposition</subject><subject>sequence analysis</subject><subject>Sequencing</subject><subject>Studies</subject><subject>Subtypes</subject><subject>Taenia</subject><subject>Trichuris trichiura</subject><subject>Urban areas</subject><subject>Zoonoses</subject><issn>1756-3305</issn><issn>1756-3305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqFkk1vFSEUhidGY2v1B7gxJG5cOHr4HjYmbf2qaWJidE0YBm65mRmuwJhcf72Mt9bWjSzgwHl5wjm8TfMUwyuMO_E6YwoSt4BlSxjvWrjXHGPJRUsp8Pu34qPmUc5bAAGKi4fNEVEgATg9bsa3IZcU-qWEOKPo0dlocol2n0vIKC992e9cRiHH0RQ3IJ_ihK6Wycz5EJsZLamvs43TtMyh7FGY0ZcQ0eDQJzO7kOJLdJbMzzA-bh54M2b35Ho9ab69f_f1_GN7-fnDxfnpZWs5Z6UdQBKQ3HSc9qYXVnhOBm98L8ATYaBmGGFKSi8NtoMUmCpFunUPvPeKnjQXB-4QzVbvUphM2utogv59ENNGm1SCHZ1WkgN2XlpiORMcFKGMOic60fUMS1dZbw6s3dJPbrBuLsmMd6B3M3O40pv4Q3PBZW14Bby4BqT4fXG56Clk68ax9iYuWRPAQOrg-L9SXH8PlGCSVenzf6TbuKS5drWqBEgFlfdXtTG11jD7WJ9oV6g-5RgzySVZVfigsinmnJy_qQ6DXo2mD0bT1Wh6NZpeq3p2uy03N_44i_4CJG_Mjg</recordid><startdate>20171025</startdate><enddate>20171025</enddate><creator>Valença Barbosa, Carolina</creator><creator>de Jesus Batista, Rosemary</creator><creator>Pereira Igreja, Ricardo</creator><creator>d'Avila Levy, Claudia Masini</creator><creator>Werneck de Macedo, Heloisa</creator><creator>Carneiro Santos, Helena Lúcia</creator><general>BioMed Central Ltd</general><general>BioMed 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Rio de Janeiro, Brazil</title><author>Valença Barbosa, Carolina ; de Jesus Batista, Rosemary ; Pereira Igreja, Ricardo ; d'Avila Levy, Claudia Masini ; Werneck de Macedo, Heloisa ; Carneiro Santos, Helena Lúcia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-d072075a853bab6c6f52dfafb60f26a05a8424977f7a1cd7613992877f705bf93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Arthritis</topic><topic>Blastocystis</topic><topic>Brazil</topic><topic>Communities</topic><topic>Deoxyribonucleic acid</topic><topic>Developing countries</topic><topic>digestive tract</topic><topic>Distribution</topic><topic>DNA</topic><topic>DNA sequences</topic><topic>DNA sequencing</topic><topic>Entamoeba</topic><topic>Enterobius vermicularis</topic><topic>Environmental health</topic><topic>Epidemiology</topic><topic>Gastrointestinal system</topic><topic>Gastrointestinal tract</topic><topic>genes</topic><topic>Genetic diversity</topic><topic>Genetic variation</topic><topic>Heterokonts</topic><topic>Human populations</topic><topic>humans</topic><topic>Identification</topic><topic>Infections</topic><topic>Irritable bowel syndrome</topic><topic>Laboratories</topic><topic>LDCs</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Nucleic acids</topic><topic>Nucleotide sequence</topic><topic>Parasites</topic><topic>Pavlova</topic><topic>PCR</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Primers</topic><topic>protists</topic><topic>Reptiles & amphibians</topic><topic>Ribonucleic acid</topic><topic>ribosomal RNA</topic><topic>RNA</topic><topic>rRNA</topic><topic>Sedimentation & deposition</topic><topic>sequence analysis</topic><topic>Sequencing</topic><topic>Studies</topic><topic>Subtypes</topic><topic>Taenia</topic><topic>Trichuris trichiura</topic><topic>Urban areas</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valença Barbosa, Carolina</creatorcontrib><creatorcontrib>de Jesus Batista, Rosemary</creatorcontrib><creatorcontrib>Pereira Igreja, Ricardo</creatorcontrib><creatorcontrib>d'Avila Levy, Claudia Masini</creatorcontrib><creatorcontrib>Werneck de Macedo, Heloisa</creatorcontrib><creatorcontrib>Carneiro Santos, Helena Lúcia</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest 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vectors</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valença Barbosa, Carolina</au><au>de Jesus Batista, Rosemary</au><au>Pereira Igreja, Ricardo</au><au>d'Avila Levy, Claudia Masini</au><au>Werneck de Macedo, Heloisa</au><au>Carneiro Santos, Helena Lúcia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil</atitle><jtitle>Parasites & vectors</jtitle><addtitle>Parasit Vectors</addtitle><date>2017-10-25</date><risdate>2017</risdate><volume>10</volume><issue>1</issue><spage>518</spage><epage>518</epage><pages>518-518</pages><artnum>518</artnum><issn>1756-3305</issn><eissn>1756-3305</eissn><abstract>Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil.
A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers.
Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%).
DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>29070053</pmid><doi>10.1186/s13071-017-2458-0</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Arthritis Blastocystis Brazil Communities Deoxyribonucleic acid Developing countries digestive tract Distribution DNA DNA sequences DNA sequencing Entamoeba Enterobius vermicularis Environmental health Epidemiology Gastrointestinal system Gastrointestinal tract genes Genetic diversity Genetic variation Heterokonts Human populations humans Identification Infections Irritable bowel syndrome Laboratories LDCs Medical research Medicine, Experimental Nucleic acids Nucleotide sequence Parasites Pavlova PCR Phylogeny Polymerase chain reaction Primers protists Reptiles & amphibians Ribonucleic acid ribosomal RNA RNA rRNA Sedimentation & deposition sequence analysis Sequencing Studies Subtypes Taenia Trichuris trichiura Urban areas Zoonoses |
title | Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil |
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