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Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil

Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small s...

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Published in:Parasites & vectors 2017-10, Vol.10 (1), p.518-518, Article 518
Main Authors: Valença Barbosa, Carolina, de Jesus Batista, Rosemary, Pereira Igreja, Ricardo, d'Avila Levy, Claudia Masini, Werneck de Macedo, Heloisa, Carneiro Santos, Helena Lúcia
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cited_by cdi_FETCH-LOGICAL-c554t-d072075a853bab6c6f52dfafb60f26a05a8424977f7a1cd7613992877f705bf93
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container_title Parasites & vectors
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creator Valença Barbosa, Carolina
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Carneiro Santos, Helena Lúcia
description Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil. A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers. Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%). DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro.
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Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil. A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers. Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%). DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. 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Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil. A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers. Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%). DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>29070053</pmid><doi>10.1186/s13071-017-2458-0</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
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source Publicly Available Content Database; PubMed Central
subjects Animals
Arthritis
Blastocystis
Brazil
Communities
Deoxyribonucleic acid
Developing countries
digestive tract
Distribution
DNA
DNA sequences
DNA sequencing
Entamoeba
Enterobius vermicularis
Environmental health
Epidemiology
Gastrointestinal system
Gastrointestinal tract
genes
Genetic diversity
Genetic variation
Heterokonts
Human populations
humans
Identification
Infections
Irritable bowel syndrome
Laboratories
LDCs
Medical research
Medicine, Experimental
Nucleic acids
Nucleotide sequence
Parasites
Pavlova
PCR
Phylogeny
Polymerase chain reaction
Primers
protists
Reptiles & amphibians
Ribonucleic acid
ribosomal RNA
RNA
rRNA
Sedimentation & deposition
sequence analysis
Sequencing
Studies
Subtypes
Taenia
Trichuris trichiura
Urban areas
Zoonoses
title Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil
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