Efficacy of T Regulatory Cells, Th17 Cells and the Associated Markers in Monitoring Tuberculosis Treatment Response

Treatment monitoring is an essential aspect for tuberculosis (TB) disease management. Sputum smear microscopy is the only available tool for monitoring, but it suffers from demerits. Therefore, we sought to evaluate markers and cellular subsets of T regulatory (Treg) cells and T helper (Th) 17 cells...

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Bibliographic Details
Published in:Frontiers in immunology 2018-02, Vol.9, p.157-157
Main Authors: Agrawal, Sonali, Parkash, Om, Palaniappan, Alangudi Natarajan, Bhatia, Ashok Kumar, Kumar, Santosh, Chauhan, Devendra Singh, Madhan Kumar, M
Format: Article
Language:English
Subjects:
Adult
Antitubercular Agents - therapeutic use
Biomarkers - analysis
Cells, Cultured
Drug Monitoring - methods
Female
Flow Cytometry
Humans
Immunology
Interleukin-2 Receptor alpha Subunit - genetics
Leukocytes, Mononuclear - drug effects
Male
Middle Aged
monitoring
T-Lymphocytes, Regulatory - immunology
Th17
Th17 Cells - immunology
treatment
Treatment Outcome
Treg
Tuberculin - pharmacology
tuberculosis
Tuberculosis, Pulmonary - drug therapy
Tuberculosis, Pulmonary - immunology
Young Adult
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Summary:Treatment monitoring is an essential aspect for tuberculosis (TB) disease management. Sputum smear microscopy is the only available tool for monitoring, but it suffers from demerits. Therefore, we sought to evaluate markers and cellular subsets of T regulatory (Treg) cells and T helper (Th) 17 cells in pulmonary TB patients (PTB) for TB treatment monitoring. Peripheral blood mononuclear cells (PBMCs) were stimulated (with purified protein derivative (PPD)) overnight which was followed by a polychromatic flow cytometry approach to study Treg and Th17 markers and cellular subsets in PTB (  = 12) undergoing antituberculous treatment (ATT). The baseline levels of these markers and cellular subsets were evaluated in normal healthy subjects (NHS). We observed a significant decrease in the expression of CD25 (
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2018.00157