Nanoscale separations: Recent achievements

• Published in: Journal of Chromatography Open 2022-11, Vol.2, p.100066, Article 100066
• Main Authors: Aydoğan, Cemil, Beltekin, Büşra, Aslan, Hakiye, Yılmaz, Fatma, Göktürk, Ilgım, Denizli, Adil, El-Rassi, Ziad
• Format: Article
• Language: English
• Subjects: Capillary electrophoresis; Chip; Food analysis; Mass spectrometry; Miniaturization; Monolith; Nano-liquid chromatography; Proteomics
• ISSN: 2772-3917; 2772-3917
• DOI: 10.1016/j.jcoa.2022.100066

•Nano-scale separation techniques (NSTs) are key tools in sensitive bioanalytical methods.•Recent overview in NSTs is presented.•Recent and selected applications of molecular separation-analyses by means of NSTs are reviewed.•Nano-column technology is presented and discussed.•The most recent achievements based on NSTs are discussed. Nanoscale separation techniques (Capillary/nano-LC, CE, CEC and Chip based LC/CE/CEC) present a prospective trend in modern analytical chemistry due to allowing high speed analysis, low reagent consumption, high separation efficiency and green chemistry. These techniques are miniaturized techniques offering unique advantages over conventional ones in bioanalytical science and technology, and are gaining ground in a wide research area (e.g. omics, foods, cell structures). These systems are also promising tools for the analysis of very limited samples, especially in omics, and forensic sciences. Focusing on nanoscale separation-based techniques, this review presents recent research on the use of nano-columns. Special attention is given to recent achievements based on nanoscale separation techniques while their potentiality is discussed. Some recent and selected applications with or without mass spectrometry (e.g., low resolution/high resolution -MS) in the period of 2019-to present are also reported. Graphical Abstract. [Display omitted] .