Loading…
A Sesquiterpene Synthase from the Endophytic Fungus Serendipita indica Catalyzes Formation of Viridiflorol
Interactions between plant-associated fungi and their hosts are characterized by a continuous crosstalk of chemical molecules. Specialized metabolites are often produced during these associations and play important roles in the symbiosis between the plant and the fungus, as well as in the establishm...
Saved in:
Published in: | Biomolecules (Basel, Switzerland) Switzerland), 2021-06, Vol.11 (6), p.898 |
---|---|
Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c482t-dd38c5780d90cc9033cd5d56f48ab45ae43c7bed1a1b1b413b82d17e736ecd0e3 |
---|---|
cites | cdi_FETCH-LOGICAL-c482t-dd38c5780d90cc9033cd5d56f48ab45ae43c7bed1a1b1b413b82d17e736ecd0e3 |
container_end_page | |
container_issue | 6 |
container_start_page | 898 |
container_title | Biomolecules (Basel, Switzerland) |
container_volume | 11 |
creator | Ntana, Fani Bhat, Wajid W. Johnson, Sean R. Jørgensen, Hans J. L. Collinge, David B. Jensen, Birgit Hamberger, Björn |
description | Interactions between plant-associated fungi and their hosts are characterized by a continuous crosstalk of chemical molecules. Specialized metabolites are often produced during these associations and play important roles in the symbiosis between the plant and the fungus, as well as in the establishment of additional interactions between the symbionts and other organisms present in the niche. Serendipita indica, a root endophytic fungus from the phylum Basidiomycota, is able to colonize a wide range of plant species, conferring many benefits to its hosts. The genome of S. indica possesses only few genes predicted to be involved in specialized metabolite biosynthesis, including a putative terpenoid synthase gene (SiTPS). In our experimental setup, SiTPS expression was upregulated when the fungus colonized tomato roots compared to its expression in fungal biomass growing on synthetic medium. Heterologous expression of SiTPS in Escherichia coli showed that the produced protein catalyzes the synthesis of a few sesquiterpenoids, with the alcohol viridiflorol being the main product. To investigate the role of SiTPS in the plant-endophyte interaction, an SiTPS-over-expressing mutant line was created and assessed for its ability to colonize tomato roots. Although overexpression of SiTPS did not lead to improved fungal colonization ability, an in vitro growth-inhibition assay showed that viridiflorol has antifungal properties. Addition of viridiflorol to the culture medium inhibited the germination of spores from a phytopathogenic fungus, indicating that SiTPS and its products could provide S. indica with a competitive advantage over other plant-associated fungi during root colonization. |
doi_str_mv | 10.3390/biom11060898 |
format | article |
fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_9781ebe202514fcf93b0864420db8641</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_9781ebe202514fcf93b0864420db8641</doaj_id><sourcerecordid>2544598010</sourcerecordid><originalsourceid>FETCH-LOGICAL-c482t-dd38c5780d90cc9033cd5d56f48ab45ae43c7bed1a1b1b413b82d17e736ecd0e3</originalsourceid><addsrcrecordid>eNpdkl1rFDEUhgdRbKm98wcEvfHC1XzOZG6EsnS1UPCiKt6FfJzZyTKTbJOMsP56s26R1hA4h-Q5b05eTtO8JvgDYz3-aHycCcEtlr181pxTSuSKduzn80f5WXOZ8w7XJeum7GVzxjjFsmvpebO7QneQ7xdfIO0hALo7hDLqDGhIcUZlBHQdXNyPh-It2ixhu-RakSA4v_dFI18Tq9FaFz0dfkNGm5hmXXwMKA7oh0_e-WGKKU6vmheDnjJcPsSL5vvm-tv6y-r26-eb9dXtynJJy8o5Jq3oJHY9trbHjFknnGgHLrXhQgNntjPgiCaGGE6YkdSRDjrWgnUY2EVzc9J1Ue_UPvlZp4OK2qu_BzFtlU71NxOovpMEDFBMBeGDHXpmsGx5dceZGknV-nTS2i9mBmchlKSnJ6JPb4If1Tb-UtVnXs2vAm9OAjEXr7KtPtvRxhDAFkW6vhPsCL17eCXF-wVyUbPPFqZJB4hLVlRwWZsRrajo2__QXVxSqH4eKS56iQmu1PsTZVPMOcHwr2OC1XFw1OPBYX8Apii1wA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2544598010</pqid></control><display><type>article</type><title>A Sesquiterpene Synthase from the Endophytic Fungus Serendipita indica Catalyzes Formation of Viridiflorol</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Ntana, Fani ; Bhat, Wajid W. ; Johnson, Sean R. ; Jørgensen, Hans J. L. ; Collinge, David B. ; Jensen, Birgit ; Hamberger, Björn</creator><creatorcontrib>Ntana, Fani ; Bhat, Wajid W. ; Johnson, Sean R. ; Jørgensen, Hans J. L. ; Collinge, David B. ; Jensen, Birgit ; Hamberger, Björn</creatorcontrib><description>Interactions between plant-associated fungi and their hosts are characterized by a continuous crosstalk of chemical molecules. Specialized metabolites are often produced during these associations and play important roles in the symbiosis between the plant and the fungus, as well as in the establishment of additional interactions between the symbionts and other organisms present in the niche. Serendipita indica, a root endophytic fungus from the phylum Basidiomycota, is able to colonize a wide range of plant species, conferring many benefits to its hosts. The genome of S. indica possesses only few genes predicted to be involved in specialized metabolite biosynthesis, including a putative terpenoid synthase gene (SiTPS). In our experimental setup, SiTPS expression was upregulated when the fungus colonized tomato roots compared to its expression in fungal biomass growing on synthetic medium. Heterologous expression of SiTPS in Escherichia coli showed that the produced protein catalyzes the synthesis of a few sesquiterpenoids, with the alcohol viridiflorol being the main product. To investigate the role of SiTPS in the plant-endophyte interaction, an SiTPS-over-expressing mutant line was created and assessed for its ability to colonize tomato roots. Although overexpression of SiTPS did not lead to improved fungal colonization ability, an in vitro growth-inhibition assay showed that viridiflorol has antifungal properties. Addition of viridiflorol to the culture medium inhibited the germination of spores from a phytopathogenic fungus, indicating that SiTPS and its products could provide S. indica with a competitive advantage over other plant-associated fungi during root colonization.</description><identifier>ISSN: 2218-273X</identifier><identifier>EISSN: 2218-273X</identifier><identifier>DOI: 10.3390/biom11060898</identifier><identifier>PMID: 34208762</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>antifungal ; Basidiomycota ; Biosynthesis ; Colonization ; endophyte ; Endophytes ; Enzymes ; Fungi ; Genes ; Genomes ; Germination ; Metabolism ; Metabolites ; Phylogenetics ; Phytopathogenic fungi ; Piriformospora indica ; Plant resistance ; Protein biosynthesis ; Roots ; Seeds ; Serendipita indica ; Sesquiterpene synthase ; sesquiterpenoid ; Sesquiterpenoids ; Spores ; Symbionts ; Symbiosis ; terpene synthase ; Tomatoes ; Viridiflorol</subject><ispartof>Biomolecules (Basel, Switzerland), 2021-06, Vol.11 (6), p.898</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-dd38c5780d90cc9033cd5d56f48ab45ae43c7bed1a1b1b413b82d17e736ecd0e3</citedby><cites>FETCH-LOGICAL-c482t-dd38c5780d90cc9033cd5d56f48ab45ae43c7bed1a1b1b413b82d17e736ecd0e3</cites><orcidid>0000-0003-2476-805X ; 0000-0001-8261-9015 ; 0000-0002-5592-4492 ; 0000-0002-5902-6550 ; 0000-0003-2690-5820 ; 0000-0002-7049-8671 ; 000000032476805X ; 0000000182619015 ; 0000000259026550 ; 0000000270498671</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2544598010/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2544598010?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.osti.gov/biblio/1797533$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Ntana, Fani</creatorcontrib><creatorcontrib>Bhat, Wajid W.</creatorcontrib><creatorcontrib>Johnson, Sean R.</creatorcontrib><creatorcontrib>Jørgensen, Hans J. L.</creatorcontrib><creatorcontrib>Collinge, David B.</creatorcontrib><creatorcontrib>Jensen, Birgit</creatorcontrib><creatorcontrib>Hamberger, Björn</creatorcontrib><title>A Sesquiterpene Synthase from the Endophytic Fungus Serendipita indica Catalyzes Formation of Viridiflorol</title><title>Biomolecules (Basel, Switzerland)</title><description>Interactions between plant-associated fungi and their hosts are characterized by a continuous crosstalk of chemical molecules. Specialized metabolites are often produced during these associations and play important roles in the symbiosis between the plant and the fungus, as well as in the establishment of additional interactions between the symbionts and other organisms present in the niche. Serendipita indica, a root endophytic fungus from the phylum Basidiomycota, is able to colonize a wide range of plant species, conferring many benefits to its hosts. The genome of S. indica possesses only few genes predicted to be involved in specialized metabolite biosynthesis, including a putative terpenoid synthase gene (SiTPS). In our experimental setup, SiTPS expression was upregulated when the fungus colonized tomato roots compared to its expression in fungal biomass growing on synthetic medium. Heterologous expression of SiTPS in Escherichia coli showed that the produced protein catalyzes the synthesis of a few sesquiterpenoids, with the alcohol viridiflorol being the main product. To investigate the role of SiTPS in the plant-endophyte interaction, an SiTPS-over-expressing mutant line was created and assessed for its ability to colonize tomato roots. Although overexpression of SiTPS did not lead to improved fungal colonization ability, an in vitro growth-inhibition assay showed that viridiflorol has antifungal properties. Addition of viridiflorol to the culture medium inhibited the germination of spores from a phytopathogenic fungus, indicating that SiTPS and its products could provide S. indica with a competitive advantage over other plant-associated fungi during root colonization.</description><subject>antifungal</subject><subject>Basidiomycota</subject><subject>Biosynthesis</subject><subject>Colonization</subject><subject>endophyte</subject><subject>Endophytes</subject><subject>Enzymes</subject><subject>Fungi</subject><subject>Genes</subject><subject>Genomes</subject><subject>Germination</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Phylogenetics</subject><subject>Phytopathogenic fungi</subject><subject>Piriformospora indica</subject><subject>Plant resistance</subject><subject>Protein biosynthesis</subject><subject>Roots</subject><subject>Seeds</subject><subject>Serendipita indica</subject><subject>Sesquiterpene synthase</subject><subject>sesquiterpenoid</subject><subject>Sesquiterpenoids</subject><subject>Spores</subject><subject>Symbionts</subject><subject>Symbiosis</subject><subject>terpene synthase</subject><subject>Tomatoes</subject><subject>Viridiflorol</subject><issn>2218-273X</issn><issn>2218-273X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkl1rFDEUhgdRbKm98wcEvfHC1XzOZG6EsnS1UPCiKt6FfJzZyTKTbJOMsP56s26R1hA4h-Q5b05eTtO8JvgDYz3-aHycCcEtlr181pxTSuSKduzn80f5WXOZ8w7XJeum7GVzxjjFsmvpebO7QneQ7xdfIO0hALo7hDLqDGhIcUZlBHQdXNyPh-It2ixhu-RakSA4v_dFI18Tq9FaFz0dfkNGm5hmXXwMKA7oh0_e-WGKKU6vmheDnjJcPsSL5vvm-tv6y-r26-eb9dXtynJJy8o5Jq3oJHY9trbHjFknnGgHLrXhQgNntjPgiCaGGE6YkdSRDjrWgnUY2EVzc9J1Ue_UPvlZp4OK2qu_BzFtlU71NxOovpMEDFBMBeGDHXpmsGx5dceZGknV-nTS2i9mBmchlKSnJ6JPb4If1Tb-UtVnXs2vAm9OAjEXr7KtPtvRxhDAFkW6vhPsCL17eCXF-wVyUbPPFqZJB4hLVlRwWZsRrajo2__QXVxSqH4eKS56iQmu1PsTZVPMOcHwr2OC1XFw1OPBYX8Apii1wA</recordid><startdate>20210616</startdate><enddate>20210616</enddate><creator>Ntana, Fani</creator><creator>Bhat, Wajid W.</creator><creator>Johnson, Sean R.</creator><creator>Jørgensen, Hans J. L.</creator><creator>Collinge, David B.</creator><creator>Jensen, Birgit</creator><creator>Hamberger, Björn</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2476-805X</orcidid><orcidid>https://orcid.org/0000-0001-8261-9015</orcidid><orcidid>https://orcid.org/0000-0002-5592-4492</orcidid><orcidid>https://orcid.org/0000-0002-5902-6550</orcidid><orcidid>https://orcid.org/0000-0003-2690-5820</orcidid><orcidid>https://orcid.org/0000-0002-7049-8671</orcidid><orcidid>https://orcid.org/000000032476805X</orcidid><orcidid>https://orcid.org/0000000182619015</orcidid><orcidid>https://orcid.org/0000000259026550</orcidid><orcidid>https://orcid.org/0000000270498671</orcidid></search><sort><creationdate>20210616</creationdate><title>A Sesquiterpene Synthase from the Endophytic Fungus Serendipita indica Catalyzes Formation of Viridiflorol</title><author>Ntana, Fani ; Bhat, Wajid W. ; Johnson, Sean R. ; Jørgensen, Hans J. L. ; Collinge, David B. ; Jensen, Birgit ; Hamberger, Björn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-dd38c5780d90cc9033cd5d56f48ab45ae43c7bed1a1b1b413b82d17e736ecd0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>antifungal</topic><topic>Basidiomycota</topic><topic>Biosynthesis</topic><topic>Colonization</topic><topic>endophyte</topic><topic>Endophytes</topic><topic>Enzymes</topic><topic>Fungi</topic><topic>Genes</topic><topic>Genomes</topic><topic>Germination</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Phylogenetics</topic><topic>Phytopathogenic fungi</topic><topic>Piriformospora indica</topic><topic>Plant resistance</topic><topic>Protein biosynthesis</topic><topic>Roots</topic><topic>Seeds</topic><topic>Serendipita indica</topic><topic>Sesquiterpene synthase</topic><topic>sesquiterpenoid</topic><topic>Sesquiterpenoids</topic><topic>Spores</topic><topic>Symbionts</topic><topic>Symbiosis</topic><topic>terpene synthase</topic><topic>Tomatoes</topic><topic>Viridiflorol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ntana, Fani</creatorcontrib><creatorcontrib>Bhat, Wajid W.</creatorcontrib><creatorcontrib>Johnson, Sean R.</creatorcontrib><creatorcontrib>Jørgensen, Hans J. L.</creatorcontrib><creatorcontrib>Collinge, David B.</creatorcontrib><creatorcontrib>Jensen, Birgit</creatorcontrib><creatorcontrib>Hamberger, Björn</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Biomolecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ntana, Fani</au><au>Bhat, Wajid W.</au><au>Johnson, Sean R.</au><au>Jørgensen, Hans J. L.</au><au>Collinge, David B.</au><au>Jensen, Birgit</au><au>Hamberger, Björn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Sesquiterpene Synthase from the Endophytic Fungus Serendipita indica Catalyzes Formation of Viridiflorol</atitle><jtitle>Biomolecules (Basel, Switzerland)</jtitle><date>2021-06-16</date><risdate>2021</risdate><volume>11</volume><issue>6</issue><spage>898</spage><pages>898-</pages><issn>2218-273X</issn><eissn>2218-273X</eissn><abstract>Interactions between plant-associated fungi and their hosts are characterized by a continuous crosstalk of chemical molecules. Specialized metabolites are often produced during these associations and play important roles in the symbiosis between the plant and the fungus, as well as in the establishment of additional interactions between the symbionts and other organisms present in the niche. Serendipita indica, a root endophytic fungus from the phylum Basidiomycota, is able to colonize a wide range of plant species, conferring many benefits to its hosts. The genome of S. indica possesses only few genes predicted to be involved in specialized metabolite biosynthesis, including a putative terpenoid synthase gene (SiTPS). In our experimental setup, SiTPS expression was upregulated when the fungus colonized tomato roots compared to its expression in fungal biomass growing on synthetic medium. Heterologous expression of SiTPS in Escherichia coli showed that the produced protein catalyzes the synthesis of a few sesquiterpenoids, with the alcohol viridiflorol being the main product. To investigate the role of SiTPS in the plant-endophyte interaction, an SiTPS-over-expressing mutant line was created and assessed for its ability to colonize tomato roots. Although overexpression of SiTPS did not lead to improved fungal colonization ability, an in vitro growth-inhibition assay showed that viridiflorol has antifungal properties. Addition of viridiflorol to the culture medium inhibited the germination of spores from a phytopathogenic fungus, indicating that SiTPS and its products could provide S. indica with a competitive advantage over other plant-associated fungi during root colonization.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>34208762</pmid><doi>10.3390/biom11060898</doi><orcidid>https://orcid.org/0000-0003-2476-805X</orcidid><orcidid>https://orcid.org/0000-0001-8261-9015</orcidid><orcidid>https://orcid.org/0000-0002-5592-4492</orcidid><orcidid>https://orcid.org/0000-0002-5902-6550</orcidid><orcidid>https://orcid.org/0000-0003-2690-5820</orcidid><orcidid>https://orcid.org/0000-0002-7049-8671</orcidid><orcidid>https://orcid.org/000000032476805X</orcidid><orcidid>https://orcid.org/0000000182619015</orcidid><orcidid>https://orcid.org/0000000259026550</orcidid><orcidid>https://orcid.org/0000000270498671</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2218-273X |
ispartof | Biomolecules (Basel, Switzerland), 2021-06, Vol.11 (6), p.898 |
issn | 2218-273X 2218-273X |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_9781ebe202514fcf93b0864420db8641 |
source | Publicly Available Content Database; PubMed Central |
subjects | antifungal Basidiomycota Biosynthesis Colonization endophyte Endophytes Enzymes Fungi Genes Genomes Germination Metabolism Metabolites Phylogenetics Phytopathogenic fungi Piriformospora indica Plant resistance Protein biosynthesis Roots Seeds Serendipita indica Sesquiterpene synthase sesquiterpenoid Sesquiterpenoids Spores Symbionts Symbiosis terpene synthase Tomatoes Viridiflorol |
title | A Sesquiterpene Synthase from the Endophytic Fungus Serendipita indica Catalyzes Formation of Viridiflorol |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T06%3A49%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Sesquiterpene%20Synthase%20from%20the%20Endophytic%20Fungus%20Serendipita%20indica%20Catalyzes%20Formation%20of%20Viridiflorol&rft.jtitle=Biomolecules%20(Basel,%20Switzerland)&rft.au=Ntana,%20Fani&rft.date=2021-06-16&rft.volume=11&rft.issue=6&rft.spage=898&rft.pages=898-&rft.issn=2218-273X&rft.eissn=2218-273X&rft_id=info:doi/10.3390/biom11060898&rft_dat=%3Cproquest_doaj_%3E2544598010%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c482t-dd38c5780d90cc9033cd5d56f48ab45ae43c7bed1a1b1b413b82d17e736ecd0e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2544598010&rft_id=info:pmid/34208762&rfr_iscdi=true |