WHIRLY1 Occupancy Affects Histone Lysine Modification and WRKY53 Transcription in Arabidopsis Developmental Manner

Single-stranded DNA-binding proteins (SSBs) are assumed to involve in DNA replication, DNA repairmen, and gene transcription. Here, we provide the direct evidence on the functionality of an SSB, WHIRLY1, by using loss- or gain-of-function lines. We show that WHIRLY1 binding to the promoter of repres...

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Bibliographic Details
Published in:Frontiers in plant science 2018-10, Vol.9, p.1503-1503
Main Authors: Huang, Dongmei, Lan, Wei, Li, Danjing, Deng, Ban, Lin, Wenfang, Ren, Yujun, Miao, Ying
Format: Article
Language:English
Subjects:
Arabidopsis
histone modification
leaf senescence
Plant Science
ssDNA binding protein
transcription
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Summary:Single-stranded DNA-binding proteins (SSBs) are assumed to involve in DNA replication, DNA repairmen, and gene transcription. Here, we provide the direct evidence on the functionality of an SSB, WHIRLY1, by using loss- or gain-of-function lines. We show that WHIRLY1 binding to the promoter of represses the enrichment of H3K4me3, but enhances the enrichment of H3K9ac at the region contained WHIRLY1-binding sequences and TATA box or the translation start region of , coincided with a recruitment of RNAPII. ChIP assays confirm that WHIRLY1 inhibits H3K4me3 enrichment at the preinitiation complex formation stage, while promotes H3K9ac enrichment and RNAPII recruitment at the elongation stage, consequently affecting the transcription of . These results further explore the molecular actions underlying SSB-mediated gene transcription through epigenetic regulation in plant senescence.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2018.01503