Loading…
Deletion of the B125R gene in the African swine fever virus SY18 strain leads to an A104R frameshift mutation slightly attenuating virulence in domestic pigs
•We investigated the effects of B125R deletion on the virulence of ASFY-SY18 strain.•B125R deletion caused A104R frameshift mutation.•The recombinant strain slightly attenuating virulence in domestic pigs. African swine fever (ASF), caused by the ASF virus (ASFV), is a hemorrhagic and fatal viral di...
Saved in:
Published in: | Virus research 2024-05, Vol.343, p.199343-199343, Article 199343 |
---|---|
Main Authors: | , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | •We investigated the effects of B125R deletion on the virulence of ASFY-SY18 strain.•B125R deletion caused A104R frameshift mutation.•The recombinant strain slightly attenuating virulence in domestic pigs.
African swine fever (ASF), caused by the ASF virus (ASFV), is a hemorrhagic and fatal viral disease that affects Eurasian wild boars and domestic pigs, posing a substantial threat to the global pig breeding industry. ASFV, a double-stranded DNA virus, possesses a large genome containing up to 160 open reading frames, most of which exhibit unknown functions. The B125R gene of ASFV, located at the 105595–105972 bp site in the ASFV-SY18 genome, remains unexplored. In this study, we discovered that B125R deletion did not affect recombinant virus rescue, nor did it hinder viral replication during the intermediate growth phase. Although the virulence of the recombinant strain harboring this deletion was attenuated, intramuscular inoculation of the recombinant virus in pigs at doses of 102 or 104 TCID50 resulted in mortality. Moreover, sequencing analysis of six recombinant strains obtained from three independent experiments consistently revealed an adenine insertion at the 47367–47375 bp site in the A104R gene due to the B125R deletion, leading to premature termination of this gene. Intriguingly, this insertion did not influence the transcription of the A104R gene between the recombinant and parental strains. Consequently, we postulate that the deletion of the B125R gene in ASFV-SY18 or other genotype II strains may marginally attenuate virulence in domestic pigs. |
---|---|
ISSN: | 0168-1702 1872-7492 |
DOI: | 10.1016/j.virusres.2024.199343 |