A single-cell atlas of the mouse and human prostate reveals heterogeneity and conservation of epithelial progenitors

Understanding the cellular constituents of the prostate is essential for identifying the cell of origin for prostate adenocarcinoma. Here, we describe a comprehensive single-cell atlas of the adult mouse prostate epithelium, which displays extensive heterogeneity. We observe distal lobe-specific lum...

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Bibliographic Details
Published in:eLife 2020-09, Vol.9
Main Authors: Crowley, Laura, Cambuli, Francesco, Aparicio, Luis, Shibata, Maho, Robinson, Brian D, Xuan, Shouhong, Li, Weiping, Hibshoosh, Hanina, Loda, Massimo, Rabadan, Raul, Shen, Michael M
Format: Article
Language:English
Subjects:
Animals
B cells
Cancer Biology
Cells, Cultured
Epithelial Cells - classification
Epithelial Cells - cytology
epithelial heterogeneity
Humans
Male
Mice
multipotent progenitor
organoids
Organoids - cytology
Prostate - cytology
renal grafts
Single-Cell Analysis
Stem Cells - classification
Stem Cells - cytology
Stem Cells and Regenerative Medicine
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Summary:Understanding the cellular constituents of the prostate is essential for identifying the cell of origin for prostate adenocarcinoma. Here, we describe a comprehensive single-cell atlas of the adult mouse prostate epithelium, which displays extensive heterogeneity. We observe distal lobe-specific luminal epithelial populations (LumA, LumD, LumL, and LumV), a proximally enriched luminal population (LumP) that is not lobe-specific, and a periurethral population (PrU) that shares both basal and luminal features. Functional analyses suggest that LumP and PrU cells have multipotent progenitor activity in organoid formation and tissue reconstitution assays. Furthermore, we show that mouse distal and proximal luminal cells are most similar to human acinar and ductal populations, that a PrU-like population is conserved between species, and that the mouse lateral prostate is most similar to the human peripheral zone. Our findings elucidate new prostate epithelial progenitors, and help resolve long-standing questions about anatomical relationships between the mouse and human prostate.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.59465