Chlamydia pneumoniae -Induced IFN-Gamma Responses in Peripheral Blood Mononuclear Cells Increase Numbers of CD4+ but Not CD8+ T Effector Memory Cells

causes respiratory infection in adults and children. Previous studies in our laboratory identified significantly higher in vitro T lymphocyte responses to in children with asthma compared to healthy controls which may indicate the presence of T effector memory (TEM) lymphocytes. In the present study...

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Bibliographic Details
Published in:Journal of blood medicine 2021-01, Vol.12, p.385-394
Main Authors: Smith-Norowitz, Tamar A, Shidid, Sarah, Norowitz, Yitzchok M, Kohlhoff, Stephan
Format: Article
Language:English
Subjects:
Asthma
Asthma in children
Bacterial pneumonia
Biological response modifiers
Blood
c. pneumoniae
Cell culture
Chlamydia
Cytokines
Enzyme-linked immunosorbent assay
Experiments
Health aspects
Immunoglobulins
Infections
Interferon
interleukin-2
Interleukins
Laboratories
Lymphocytes
Medical research
Medicine, Experimental
Memory (Computers)
Original Research
Pneumonia
Population
Respiratory diseases
Software
T cells
t effector memory lymphocytes
Tetracycline
Tetracyclines
Tuberculosis
Viral infections
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Summary:causes respiratory infection in adults and children. Previous studies in our laboratory identified significantly higher in vitro T lymphocyte responses to in children with asthma compared to healthy controls which may indicate the presence of T effector memory (TEM) lymphocytes. In the present study, healthy subjects were screened for the presence of TEM cells and their cytokines. CCR7 negative effector TEMs may indicate persistent infection with . Peripheral blood mononuclear cells (PBMC) (1×10 /mL) from adult non-asthmatic subjects were infected for 1h ± TW-183 at a multiplicity of infection (MOI) = 0.1 and cultured (48 hrs). Distributions of lymphocytes (CD4+, CD8+) and TEM cells (CD4+CCR7+CD45RA+CD154+, CD8+CCR7+CD45RA+CD154+) were determined. Levels of intracellular interleukin (IL)-2, IL-4, and interferon (IFN)-gamma were measured (flow microfluorimetry); IFN-gamma was measured in supernatants (ELISA). infection led to a decrease in numbers of CD8+ TEM and CD8+CD154+ cells; CD4+TEM and CD4+CD154+ cells did not change. Numbers of TEM cells (CD4+IL-2+, CD8+ IL-2+) also decreased. However, number of TEM cells (CD4+IL4-+, CD8+ IL-4+) and (CD4+ IFN-gamma+, CD8+IFN-gamma+) did not change. When stratified according to IFN-gamma+ status, numbers of CD4+ IL-2+ and CD4+IL-4+ TEMs increased; CD8+IL-2+ and CD8+ IL-4+ TEMs decreased. -induced PBMC IFN-gamma+ responses increased numbers of CD4+ IL-2+ and CD4+IL-4+ TEM cells, while CD8+IL-2+ and CD8+IL-4+ TEMs decreased. Production of IFN-gamma by infected PBMC should be further studied as a biomarker of persistent infection in humans.
ISSN:1179-2736
1179-2736
DOI:10.2147/JBM.S303275