Identification and Characterization of a Luteinizing Hormone Receptor (LHR) Homolog from the Chinese Mitten Crab Eriocheir sinensis

Luteinizing hormone (LH), a pituitary gonadotropin, coupled with LH receptor (LHR) is essential for the regulation of the gonadal maturation in vertebrates. Although LH homolog has been detected by immunocytochemical analysis, and its possible role in ovarian maturation was revealed in decapod crust...

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Bibliographic Details
Published in:International journal of molecular sciences 2019-04, Vol.20 (7), p.1736
Main Authors: Yuan, Li-Juan, Peng, Chao, Liu, Bi-Hai, Feng, Jiang-Bin, Qiu, Gao-Feng
Format: Article
Language:English
Subjects:
Amino acids
Crustaceans
Decapoda
Eriocheir sinensis
expression profile
Frontotemporal dementia
Glycoproteins
Gonadotropin-releasing hormone
Homology
Hybridization
Investigations
Luteinizing hormone
luteinizing hormone receptor
Maturation
Next-generation sequencing
ovarian development
Phylogenetics
Prawns
Proteins
Signal transduction
Vertebrates
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Summary:Luteinizing hormone (LH), a pituitary gonadotropin, coupled with LH receptor (LHR) is essential for the regulation of the gonadal maturation in vertebrates. Although LH homolog has been detected by immunocytochemical analysis, and its possible role in ovarian maturation was revealed in decapod crustacean, so far there is no molecular evidence for the existence of LHR. In this study, we cloned a novel homolog (named ) from the Chinese mitten crab . The complete sequence of the cDNA was 2775bp, encoding a protein of 924 amino acids, sharing 71% amino acids identity with the ant LHR. expression was found to be high in the ovary, while low in testis, gill, brain, and heart, and no expression in the thoracic ganglion, eye stalk, muscle, and hepatopancreas. Quantitative PCR revealed that the expression level of mRNA was significantly higher in the ovaries in previtellogenic (Pvt), late vitellogenic (Lvt), and germinal vesicle breakdown (GVBD) stages than that in the vitellogenic (Mvt) and early vitellogenic (Evt) stages ( < 0.05), and, the highest and the lowest expression were in Lvt, and Evt, respectively. The strong signal was mainly localized in the ooplasm of Pvt oocyte as detected by in situ hybridization. The crab GnRH homolog can significantly induce the expression of mRNA at 36 hours post injection in vivo ( < 0.01), suggesting that may be involved in regulating ovarian development through GnRH signaling pathway in the mitten crab.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20071736