The Cell Death Triggered by the Nuclear Localized RxLR Effector PITG_22798 from Phytophthora infestans Is Suppressed by the Effector AVR3b

Phytopathogenic oomycetes, such as , potentially secrete many RxLR effector proteins into plant cells to modulate plant immune responses and promote colonization. However, the molecular mechanisms by which these RxLR effectors suppress plant immune responses are largely unknown. Here we describe an...

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Bibliographic Details
Published in:International journal of molecular sciences 2017-02, Vol.18 (2), p.409-409
Main Authors: Wang, Hongyang, Ren, Yajuan, Zhou, Jing, Du, Juan, Hou, Juan, Jiang, Rui, Wang, Haixia, Tian, Zhendong, Xie, Conghua
Format: Article
Language:English
Subjects:
Apoptosis
Cell Death
Cell Nucleus - metabolism
Colonization
effector
Effector cells
Effectors
Fluorescence
Green fluorescent protein
Host-Parasite Interactions
Immune response
Leaves
Localization
Molecular modelling
Nicotiana benthamiana
Nuclei
Nucleotide sequence
Oomycetes
Pathogens
Phytophthora infestans
Phytophthora infestans - pathogenicity
Phytophthora infestans - physiology
Plant cells
Plant immunity
Plants - parasitology
Potatoes
Protein Transport
Proteins
Protozoan Proteins - metabolism
Solanum tuberosum
Virulence Factors - metabolism
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Summary:Phytopathogenic oomycetes, such as , potentially secrete many RxLR effector proteins into plant cells to modulate plant immune responses and promote colonization. However, the molecular mechanisms by which these RxLR effectors suppress plant immune responses are largely unknown. Here we describe an RxLR effector PITG_22798 (Gene accession: XM_002998349) that was upregulated during early infection of potato by . By employment of agroinfiltration, we observed that triggers cell death in . Confocal microscopic examination showed that PITG_22798-GFP (Green Fluorescent Protein) located in the host nucleus when expressed transiently in leaves. A nuclear localization signal (NLS) domain of PITG_22798 is important for nuclear localization and cell death-inducing activity. Sequence alignment and transient expression showed that from diverse isolates are conserved, and transient expression of enhances colonization of leaves, which suggests that contributes to infection. -triggered cell death is dependent on SGT1-mediated signaling and is suppressed by the avirulence effector 3b (AVR3b). The present research provides a clue for further investigation of how effector PITG_22798 associates with and modulates host immunity.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms18020409