Prolonged-duration pulsed radiofrequency is associated with increased neuronal damage without further antiallodynic effects in neuropathic pain model rats

Pulsed radiofrequency (PRF) is a safe and effective approach for treating neuropathic pain. However, the optimal treatment conditions and analgesic mechanisms of PRF remain unclear. The aim of our study was to assess the beneficial and adverse effects of prolonged-duration PRF and the analgesic mech...

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Bibliographic Details
Published in:Journal of pain research 2018-01, Vol.11, p.2645-2651
Main Authors: Arakawa, Kyosuke, Kaku, Ryuji, Kurita, Masako, Matsuoka, Yoshikazu, Morimatsu, Hiroshi
Format: Article
Language:English
Subjects:
Analgesics
antiallodynic effects
ATF3
Electrodes
Ganglion cysts
HCN channels
Messenger RNA
Muscle contraction
Neurons
neuropathic pain
Original Research
Pain
Pain management
Polymerase chain reaction
pulsed radiofrequency
RNA
Studies
Surgery
Visualization
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Summary:Pulsed radiofrequency (PRF) is a safe and effective approach for treating neuropathic pain. However, the optimal treatment conditions and analgesic mechanisms of PRF remain unclear. The aim of our study was to assess the beneficial and adverse effects of prolonged-duration PRF and the analgesic mechanisms of PRF treatment with neuropathic pain rats. Male Sprague Dawley rats received L5 spinal nerve ligation (SNL) for developing neuropathic pain. Fourteen days after L5 SNL surgery, they were divided into three groups according to duration of PRF current for 6 minutes, 12 minutes, and none. PRF current was delivered via direct visualization adjacent to the L5 dorsal root ganglion (DRG). Pain behavior was evaluated every week after L5 SNL surgery, until day 28. Seven days after PRF treatment, L5 DRG tissue was harvested to detect levels of activating translation factor 3 (ATF3; a marker of neuronal damage) and hyperpolarization-activated cyclic nucleotide (HCN)-gated cation channels (key factors in neuropathic pain) using quantitative PCR. Before PRF application, withdrawal thresholds were significantly lower than at baseline and did not differ significantly between the three groups. After PRF application, withdrawal thresholds in the PRF6 and PRF12 groups were significantly increased compared to those in the sham group. However, those in the PRF6 and PRF12 groups did not differ significantly. The expression level of mRNA in the PRF12 group was significantly higher than that in the sham group (
ISSN:1178-7090
1178-7090
DOI:10.2147/JPR.S168064