HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection

• Published in: Scientific reports 2021-04, Vol.11 (1), p.9274-9274, Article 9274
• Main Authors: Basso, Monica, Zago, Daniela, Scaggiante, Renzo, Cavinato, Silvia, Pozzetto, Irene, Stagni, Camilla, Parisatto, Beatrice, Cattelan, Anna Maria, Battagin, Giuliana, Sarmati, Loredana, Parisi, Saverio Giuseppe
• Format: Article
• Language: English
• Subjects: 692/308; 692/420; 692/699; Antiviral agents; Antiviral Agents - therapeutic use; Archives & records; Coinfection - drug therapy; Coinfection - virology; Deoxyribonucleic acid; Disease; DNA; DNA, Viral - analysis; DNA, Viral - genetics; Female; Hepacivirus - drug effects; Hepacivirus - genetics; Hepatitis; Hepatitis C - drug therapy; Hepatitis C - virology; HIV; HIV Infections - drug therapy; HIV Infections - virology; HIV-1 - drug effects; HIV-1 - genetics; HIV-1 - physiology; Hospitals; Human immunodeficiency virus; Humanities and Social Sciences; Humans; Infections; Leukocytes (mononuclear); Leukocytes, Mononuclear - virology; Longitudinal Studies; Male; Middle Aged; multidisciplinary; Peripheral blood mononuclear cells; Plasma; Science; Science (multidisciplinary); Tropism; Viral Tropism - physiology; Viremia
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-88811-6

We described short-term HIV tropism changes occurring in peripheral blood mononuclear cells and the correlations with HIV DNA value in HIV-HCV co-infected patients cured for HCV disease and with undetectable HIV viremia or residual viremia (RV). Plasma HIV RNA, cellular HIV DNA and tropism were evaluated pre-HCV treatment (baseline, BL) and at 12(T1) and 24(T2) weeks after HCV treatment start. V3 sequences were interpreted using Geno2pheno and classified as R5 only if all three sequences had an FPR ≥ 10% and as X4 when at least one replicate sequence had an FPR