Identification of Vibrio metschnikovii and Vibrio injensis Isolated from Leachate Ponds: Characterization of Their Antibiotic Resistance and Virulence-Associated Genes

This study aimed to evaluate the antibiotic resistance of 22 environmental Vibrio metschnikovii isolates and 1 Vibrio injensis isolate from landfill leachates in southwestern Colombia. Isolates were identified by Matrix-Assisted Laser Desorption/Ionization–Time-Of-Flight (MALDI-TOF), and 16S ribosom...

Full description

Saved in:
Bibliographic Details
Published in:Antibiotics (Basel) 2023-11, Vol.12 (11), p.1571
Main Authors: Falco, Aura, Villaquirán-Muriel, Miguel Ángel, Gallo Pérez, José David, Mondragón-Quiguanas, Alejandra, Aranaga, Carlos, Correa, Adriana
Format: Article
Language:English
Subjects:
Aminoglycosides
Antibacterial agents
antibiotic
Antibiotic resistance
Antibiotics
Antimicrobial agents
Aquatic environment
Bacteria
beta-lactams
Biofilms
Ciprofloxacin
Drug resistance
Flagella
Gene sequencing
Genes
Genomes
Hemolysins
Identification
Imipenem
Infections
Ionization
Landfill
Landfills
Leachates
Multidrug resistance
resistance
rRNA 16S
Vibrio
Vibrio injensis
Vibrio metschnikovii
Virulence
Waste disposal sites
β-Lactam antibiotics
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aimed to evaluate the antibiotic resistance of 22 environmental Vibrio metschnikovii isolates and 1 Vibrio injensis isolate from landfill leachates in southwestern Colombia. Isolates were identified by Matrix-Assisted Laser Desorption/Ionization–Time-Of-Flight (MALDI-TOF), and 16S ribosomal RNA gene sequencing. Analysis of the susceptibility to six antibacterial agents by the Kirby–Bauer method showed susceptibility of all the isolates to ciprofloxacin and imipenem. We recorded resistance to beta-lactams and aminoglycosides, but no multidrug resistance was observed. The genome of one of the isolates was sequenced to determine the pathogenic potential of V. injensis. Genes associated with virulence were identified, including for flagellar synthesis, biofilm formation, and hemolysins, among others. These results demonstrate that landfill leachates are potential reservoirs of antibiotic-resistant and pathogenic bacteria and highlight the importance of monitoring Vibrio species in different aquatic environments.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics12111571