The detection and subsequent volume optimization of biological nanocrystals

Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second...

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Bibliographic Details
Published in:Structural dynamics (Melville, N.Y.) N.Y.), 2015-07, Vol.2 (4), p.041710-041710
Main Authors: Luft, Joseph R, Wolfley, Jennifer R, Franks, Eleanor Cook, Lauricella, Angela M, Gualtieri, Ellen J, Snell, Edward H, Xiao, Rong, Everett, John K, Montelione, Gaetano T
Format: Article
Language:English
Subjects:
Crystal structure
Crystallization
Crystallography
Crystals
Experiments
Laboratories
Microcrystals
Microscopy
Molecular structure
Nanocrystals
Optimization
Phase diagrams
Proteins
SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2
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Summary:Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second-order nonlinear imaging of chiral crystals and ultraviolet two-photon excited fluorescence detection is shown to be applicable in a high-throughput manner to rapidly verify the presence of nanocrystals in crystallization screening conditions. It is noted that the nanocrystals are rarely seen without also producing microcrystals from other chemical conditions. A crystal volume optimization method is described and associated with a phase diagram for crystallization.
ISSN:2329-7778
2329-7778
DOI:10.1063/1.4921199