High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers

Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification...

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Bibliographic Details
Published in:BMC genomics 2019-01, Vol.20 (1), p.59-59, Article 59
Main Authors: Gustafsson, Charlotte, De Paepe, Ayla, Schmidl, Christian, Månsson, Robert
Format: Article
Language:English
Subjects:
Bioinformatics
ChIP-seq
ChIPmentation
Chromatin
Chromatin immunoprecipitation
Crosslinking
Deoxyribonucleic acid
DNA
EDTA
Enzymes
Epigenetic inheritance
Epigenetics
Flow cytometry
Genomes
Genomics
High-throughput genomics
High-throughput screening (Biochemical assaying)
Immunoprecipitation
Libraries
Medicin och hälsovetenskap
Methodology
Methods
Molecular biology
Polymer crosslinking
Proteins
Purification
Quality
Transcription (Genetics)
Transcription factors
Workflow
Workflow software
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Summary:Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-018-5299-0